Lallemand Hungary Liquidity Management LLC

Stellenbosch University

Mascoma Corporation

Hydrojoule, LLC

Universiteit Stellenbosch

Elena E Brevnova

John E McBride

Mark Mellon

Vineet Rajgarhia

Erin Wiswall

Anne K Warner

Chhayal V Gandhi

Allan C Froehlich

Nicky Caiazza

Riaan Den Haan

Emily Stonehouse

Shital A Tripathi

Haowen Xu

Kristen M Deleault

John S Bardsley

Aaron Argyros

Trisha Barrett

Charles F Rice

Ryan Skinner

Frank Agbogbo

Kevin S Wenger

Indraneel Shikhare

Alan Belcher

Vineet B Rajgarhia

Abigail Foster

Jennifer Gosselin

Yin-Ying Chiu

Heidi H Hau

Merja Elisa Penttila

Matti Siika-Aho

Arthur J Shaw, Iv

Johannes Pieter Van Dijken

Marja Ilmen

Alan Benjamin Gilbert

William Ryan Sillers

James H Flatt

Sanni Voutilainen

James Justin Lancaster

Jaana Uusitalo

Anu Koivula

Deidre Willes

John Mcbride

David Anthony Hogsett

Emile Van Zyl

Shaunita Hellouise Rose

Danie Lagrange

Jim Flatt

Heidi Lau

Shaunita Rose

The present invention provides for novel metabolic pathways leading to propanol, alcohol or polyol formation in a consolidated bioprocessing system (CBP), where lignocellulosic biomass is efficiently converted to such products. More specifically, the invention provides for a recombinant microorganism, where the microorganism expresses one or more native and/or heterologous enzymes; where the one or more enzymes function in one or more engineered metabolic pathways to achieve: (1) conversion of a carbohydrate source to 1,2-propanediol, isopropropanol, ethanol and/or glycerol; (2) conversion of a carbohydrate source to n-propanol and isopropanol; (3) conversion of a carbohydrate source to isopropanol and methanol; or (4) conversion of a carbohydrate source to propanediol and acetone; wherein the one or more native and/or heterologous enzymes is activated, upregulated or downregulated.

Production of propanols, alcohols, and polyols in consolidated bioprocessing organisms

The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.

Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose

The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.

Heterologous expression of termite cellulases in yeast

The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterologous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability ofto produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.

Yeast expressing cellulases for simultaneous saccharification and fermentation using cellulose

The present invention relates to the engineering and expression of heterologous cellulosomes in microorganisms in order to facilitate the conversion of biomass to useful products. In some embodiments, the invention relates to the expression of scaffoldin proteins which form the nucleus of a cellulosome. Cellulases or other biomass-degrading enzymes can be non-covalently linked to the scaffoldin protein by virtue of a dockerin domain-cohesin domain interaction.

Yeast cells expressing an exogenous cellulosome and methods of using the same

Host cells, comprisingexpressing heterologous cellulases produce ethanol from cellulose In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose The recombinant yeast strains and co-cultures of the yeast strains can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of sacchanfication and fermentation processes.

Heterologous expression of termite cellulases yeast

An online business method and system enables donors or parents or guardians of donors to order and purchase stem-cells extracted from biological tissue sampled from the donor, such as, for example, cord-blood stem cells of a newborn baby, wherein the ordering process interfaces directly with the attending medical services, and the service steps include collection, extraction, preservation, containment, packaging, delivery and storage of the stem cells in a storage medium that can be cost-effectively maintained in a storage facility or by the donor, parent or guardian at home. In one embodiment, preservation is by freeze-drying, containment is in a vacuum vial, and storage is at room temperature.

Business method and system for ordering, purchasing and storing stem cells

The present invention provides for the isolation and characterization of the cbh1 gene from. In particular, the present invention provides for the nucleic acid and amino acid sequences ofcbh1, and domains, variants and derivatives thereof. The present invention further provides for the heterologous expression ofCbh1 in host cells, including yeast, e.g.,. Expression ofCbh1 in host cells will augment cellulose digestion and facilitate ethanol production by those host cells on cellulosic substrates. In certain embodiments, heterologous expression inis in coordination with heterologous expression of other known, or newly identified saccharolytic enzymes. Therefore, the present invention also provides that the novelCbh1 gene can utilized in a consolidated bioprocessing system.

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Winchester, MA, United States of America

Elena E Brevnova