Illumina Cambridge Limited

Elena Cressina

Xiaohai Liu

Antoine Francais

Angelica Mariani

Adam Culley

Xiaolin Wu

Anno Koetje

Geoffrey Paul Smith

Wouter Meuleman

Rosamond Jackson

Embodiments of the present disclosure relate to nucleotide with acetal 3'-OH blocking groups. Also provided herein are methods of using fully functionalized nucleotides containing the 3′ acetal blocking group for sequencing applications.

Methods of sequencing using nucleotides with 3' acetal blocking group

Embodiments of the present disclosure relate to nucleotide molecules with a 3' AOM blocking group. Also provided herein are methods to prepare such nucleotide molecules, and the uses of fully functionalized nucleotides containing the 3′-OH blocking group for sequencing applications.

Nucleotides with a 3' AOM blocking group

The present disclosure relates to new nucleotide and oligonucleotide compounds and their use in nucleic acid sequencing applications.

Short pendant arm linkers for nucleotides in sequencing applications

Embodiments of the present disclosure relate to methods of preparing growing polynucleotides using nucleotide molecules with a 3' AOM blocking group. Also provided herein are kits related to such methods.

Methods of preparing growing polynucleotides using nucleotides with 3' AOM blocking group

Embodiments of the present disclosure relate to nucleotide and nucleoside molecules with acetal 3'-OH blocking groups. Also provided herein are methods to prepare such nucleotide and nucleoside molecules, and the uses of fully functionalized nucleotides containing the 3&#x2032; acetal blocking group for sequencing applications.

Nucleosides and nucleotides with 3' acetal blocking group

Embodiments of the present disclosure relate to sequencing methods using nucleotide molecules with a 3' AOM blocking group, which can provide reduced phasing and pre-phasing values. Also provided herein are kits related to such sequencing methods.

Sequencing methods using nucleotides with 3' AOM blocking group

A reaction mixture including (a) a nucleic acid having a primer hybridized to a template, (b) nucleotide analogs, wherein the nucleotide analogs have a blocking moiety; (c) a polymerase that is capable of forming an extended primer by adding the nucleotide analogs to the primer, and (d) a deblocking agent that is capable of removing the blocking moiety from the extended primer. Also provided is a method of synthesizing a polynucleotide including sequentially adding a plurality of the different nucleotides analogs to the nucleic acid via several reaction cycles in the reaction mixture, wherein each reaction cycle includes (i) the polymerase adding a nucleotide analog to the nucleic acid to form a transient nucleic acid species comprising a blocking moiety, and (ii) the deblocking agent modifying the transient nucleic acid species to remove the blocking moiety.

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Cambridge, United Kingdom

Elena Cressina