Stellenbosch University

Danstar Ferment Ag

Lallemand Hungary Liquidity Management LLC

Universiteit Stellenbosch

Elena Brevnova

Vineet Rajgarhia

Aaron Argyros

Trisha Barrett

Nicky Caiazza

Merja Penttilä

Alan Benjamin Gilbert

Deidre Willes

Naomi Thorngren

Jim Flatt

Jaana Uusitalo

Danie Lagrange

Shaunita Rose

Marja Ilmen

Matti Siika-Aho

Merja Penttila

Vineet B Rajgarhia

Abigail S Foster

Alan Belcher

Chhayal Ghandi

Jeffrey D Keating

Emily A Stonehouse

Heidi Hanson Hau

Dan Murphy

Alan Froehlich

Jeff Villari

John E McBride

Charles F Rice

Allan C Froehlich

Erin Wiswall

Mark Mellon

Ryan Skinner

Anne K Warner

Riaan Den Haan

Emily Stonehouse

Chhayal V Gandhi

Shital A Tripathi

Haowen Xu

Kristen M Deleault

John S Bardsley

Heidi H Hau

Kevin S Wenger

Indraneel Shikhare

Frank Agbogbo

Jennifer Gosselin

Yin-Ying Chiu

Emile Van Zyl

Abigail Foster

John E Mcbride

The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.

Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose

Growing community of inventors

Lebanon, NH, United States of America

Elena Brevnova