Life Technologies Corporation

Applied Biosystems, LLC

Other

Pe Corporation

Apptera Corporation

Elana Swartzman

Linda G Lee

Ronald J Graham

William E Werner

Lily Lu

Stephen P Hendricks

David Ruff

Shiaw-Min Chen

Mark Shannon

Julia Lu

Nivedita Sumi Majumdar

Harrison Leong

A proximity binding assay (PBA) is performed on at least one test sample, at least one reference sample, a background sample, and one or more calibration samples using a thermal cycler instrument. Ct values are determined for at least one set of test sample data and at least one set of reference sample data. Background corrected Ct values are calculated using a corresponding value in a background sample data set. A linear range is determined for the background corrected Ct values as a function of sample quantity. A linear regression line is calculated for each linear range. One or more parameter values of an exponential model (EM) fold change formula are estimated from the one or more sets of calibration sample data. A target protein quantity and associated confidence interval are calculated using the linear regression lines and the EM fold change formula.

Systems and methods for the analysis of proximity binding assay data

This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.

Workflow for detection of ligands using nucleic acids

The present invention provides novel, water-soluble, red-emitting fluorescent rhodamine dyes and red-emitting fluorescent energy-transfer dye pairs, as well as labeled conjugates comprising the same and methods for their use. The dyes, energy-transfer dye pairs and labeled conjugates are useful in a variety of aqueous-based applications, particularly in assays involving staining of cells, protein binding, and/or analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.

Water-soluble rhodamine dyes conjugates

Water-soluble rhodamine dye conjugates

Water-soluble rhodamine dye peptide conjugates

Water-soluble rhodamine dyes and conjugates thereof

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Alameda, CA, United States of America

Elana Swartzman