Technion Research & Development Foundation Limited

Bio-rad Laboratories, Inc.

Elad Brod

Uri Sivan

Aran Paulus

Roumen Bogoev

Anton Posch

Camille M Diges

Sricharan Bandhakavi

Annett Hahn-Windgassen

Itai Yanai

Tamar Hashimshony

Anatoly Senderovich

Inbal Zafir-Lavie

Annett Hanh-Windgassen

Provided are methods and systems for parallel analysis of a single cell's nucleic acid.

System and method for single cell genetic analysis

According to an aspect of some embodiments, an apparatus for isolation and cytometric analysis of cells from a liquid medium is provided. The apparatus comprises a cell reservoir, for receiving a liquid medium containing a suspension of cells. The apparatus further comprises a cell cage array between the cell reservoir and an enclosed reservoir, each cell cage comprising a large opening adjacent to the first cell reservoir, a cell cage, and one or more small opening adjacent to the enclosed reservoir. A connected liquid medium pump may move the suspended cells into cell cages by flowing the liquid medium from the cell reservoir to the enclosed reservoir. The apparatus further comprises a mechanical element which when actuated, pushes the cell cage array against a gel layer, forming a contiguous barrier to isolate each cell in a cell cage, and allowing cytometric analysis of isolated cells.

Method and apparatus for cell isolation, growth, replication, manipulation, and analysis

A specified proton concentration in a volume () is produced by passing a controlled electrophoresis current through an adjacent electrophoresis volume () between a working electrode () and a counter electrode (). An array of such volumes with specified proton concentration is used to provide the pH gradient for isoelectric focusing.

PH gradients controlled by electrolysis, and their use in isoelectric focusing

Methods and devices for purifying, detecting, and collecting analytes fractionated based on pI, separating analytes via electrophoresis and pI, and purifying a target molecule using pI focusing and subsequent crystallization are provided.

Affinity methods and compositions employing electronic control of pH

Apparatuses and methods for controlling ionic strength and/or pH of a solution are provided.

Electronic control of pH and ionic strength

Apparatuses and methods for purifying proteins and other target molecules based on pI are provided.

Electronic protein fractionation

pH gradients controlled by electrolysis, and their use in isoelectric focusing

Methods and devices for purification of different cell components from the same sample are provided.

Simultaneous purification of cell components

A method for a tactile presentation of perceivable content. The method comprises receiving a data representing perceivable content, selecting a plurality of electric currents according to the data, each the electric current is associated with at least one of a plurality of regions of a solution having a plurality of macromolecules, and changing a level of acidity (pH) of at least one of the plurality of regions by applying a respective the electric current thereto. A proton concentration in the plurality of regions tactilely presents the perceivable content.

Method and device for tactile presentation

A device for isoelectric focusing. The device comprises a focusing container configured to contain an electrolyte solution and having a longitudinal axis and at least one electrolysis unit mounted in a close proximity to the longitudinal axis. Each electrolysis unit injects an ion flow into the focusing container so as to create a pH gradient having a plurality of steps in the electrolyte solution, along the longitudinal axis. Each step has a substantially uniform pH level and the pH gradient is defined by at least one pH ramp between every two sequential steps of the plurality of steps.

Proton concentration topographies, methods and devices for producing the same

A method of separating a mixture of a plurality of molecular analytes having different isoelectric points (pIs). The method comprises placing a solution containing a mixture of a plurality of molecular analytes in a separation volume, generating a pH profile having a plurality of pH zones across an axis of the separation volume, and adjusting a profile of the pH profile to induce a migration of a first molecular analyte along the axis apart from a second molecular analyte. The first and second molecular analytes having different pIs.

Methods and devices of separating molecular analytes

Disclosed are methods for controlling molecular interactions where at least one member of a pair of chemical entities having a pH-dependent affinity is immobilized on the surface of an electrode. By changing the potential difference between the electrode and a counter electrode, the affinity of the pair of chemical entities is changed, changing the tendency of the pair to bind or to dissociate. Disclosed are also devices useful for implementing the methods of the present invention.

Growing community of inventors

Tivon, Israel

Elad Brod