Snow Brand Milk Products Co., Ltd.

Other

Daiichi Pharmaceutical Co., Ltd.

Eitaro Kumazawa

Yozo Ishioka

Yukihiro Saiki

Masatsugu Ueda

Shinjiro Mitsuda

Yoshiaki Matsuda

Atushi Baba

Fumindo Kai

Kanji Higashio

Akihiko Murakami

Katsumi Tanaka

Takashi Mano

Takao Hatai

Seiji Ishida

Masahiko Takahashi

Naoki Kobayashi

Hideo Sakuma

Masakatsu Uesaka

Nobuyuki Kawashima

Hitoshi Hayasaka

Kazuyoshi Ishida

The invention relates to a lyophilized HGF preparation prepared by lyophilizing an aqueous solution containing HGF, and a lyophilized HGF preparation containing a stabilizer, sodium chloride, a buffer, and/or a surface active agent. According to the invention, HGF can be stabilized, and it can be stored for a long period.

Lyophilized HGF preparations

This invention provides pharmaceutical preparations containing highly concentrated tumor cytotoxic factor (TCF). The pharmaceutical preparations of the present invention contain highly concentrated TCF and a basic amino acid or their salts, or an organic or inorganic salt as a solubizer. The resultant compositions described in the present invention dissolve TCF at high concentrations of 10 mg/ml or over under about neutral and isotonic conditions. The resultant compositions are stable and are suitable for injection preparations.

Highly concentrated TCF pharmaceutical preparations

A process and system for cultivating adhesive cells in a packed bed of solid cell matrix is disclosed. Adhesive cells are adhered to a solid cell matrix, preferably ceramic particles, and packed in a cell culture tank to form the packed bed. The culture medium is circulated through the packed bed in a direction. The circulating of the culture medium is then stopped and the direction of the medium being circulated is reversed. This periodic switching of the direction of the culture medium reduces channelling of the culture medium. The bottom or head of the culture tank can also contain a separator to trap any bubble existing in the culture medium or to trap any quantity of cell matrix possibly running out from the culture tank.

Process for cultivating adhesive cells in a packed bed of solid cell

Cell cultivation is carried out by proliferating cells in a small preliminary culture tank containing immobilizing carriers, stripping proliferated cells from the carriers, forming a cell suspension of the stripped cells in culture medium in an intermediate reservoir, intermittently transferring the suspension into a main culture tank containing ceramic immobilizing carriers and further culturing the cells. The main culture tank has an inversed funnel-shaped inlet above the carriers with a perforated plate attached to the bottom of the inlet for uniform distribution of cell suspension from above the carriers, and a plate in the form of a net under the carriers on a perforated plate for uniform distribution of cell suspension from beneath the carriers. The main tank additionally has a lid detachably carrying the inversed funnel-shaped inlet an intermediate drum-shaped portion from which the lid is detachable and a lower portion detachably carrying the plate in the form of a net and being detachable from the drum-shaped portion. The lower portion is held on or separated from the drum-shaped portion by means of a link mechanism and a cylinder capable of lowering the lower portion to separate it from the drum-shaped portion.

Method and apparatus for cell culture with immobilizing carriers

There is provided between a concentrate tank and a membrane filter a circulating line which consists of a feed line and a return line. It is also possible to use an ultrafilter as the membrane filter. There is provided a leveling tube connected by respective pipes to the concentrate tank at top and bottom portions thereof, respectively. A buoy floats within the leveling tube and a position of this buoy is detected by means of transparency of the leveling tube itself or photosensors in order to detect a degree of concentration of the culture fluid progressing within the concentrate tank. There may be provided multi-stage circulating lines each connecting the associated concentrate tank to the associated membrane filter so that the culture fluid can be transferred during the process of concentration from the stage of the largest volume treatment to the state of the least volume treatment in order to concentrate the culture fluid more efficiently.

Culture fluid concentrator

Here is disclosed a cell culture tank comprising a cylindrical screen mounted upright within the cell culture tank and having upper and lower openings through which culture medium flows, a pipe for supply of oxygen or oxygen containing gas and an agitator adapted to produce a flow of the culture medium, both the pipe and the agitator being provided within the cylindrical screen, wherein the agitator drives the culture medium to flow downward through the lower opening of the cylindrical screen, then to flow upward into a space defined between the cylindrical screen and the inner wall of the cell culture tank, and a relationship between an ascending velocity of the culture medium and a sinking velocity of the adherent carriers produces in the space between the cylindrical screen and the inner wall of the cell culture tank a floating region within which the adherent carriers float in the culture medium and a separating region within which the adherent carriers are separated from the culture medium.

Cell culture tank

Crushing/discharging device for materials being dried in a multistage

An improved apparatus for supplying materials, useful for belt type continuous vacuum dryer, is provided which comprises an inner cylinder equipped with a material-supplying duct fixed onto one end thereof, a distributing chamber formed inside the cylinder and communicating with the duct and a plurality of distributing nozzles fixed thereto; an outer cylinder having the inner cylinder rotatably inserted thereinto and having a plurality of distributing nozzles provided at the opposed locations to the former distributing nozzles; a screw feeder provided in freely rotatable manner inside the distributing chamber; and a driving mechanism for rotating the outer cylinder. According to this apparatus, it is possible to supply materials on a moving belt, continuously and uniformly over the whole surface of the belt.

Apparatus for supplying materials to belt type continuous vacuum dryer

A material feed pipe for use in a belt-system continuous vacuum drier and oriented above a belt moving within a vacuum drying chamber. The feed pipe includes an inner tube having its one end provided with an inlet for material and the other end being closed. An outer tube has its two ends closed and encircling said inner tube with a radial space therebetween. A plurality of nozzle openings are provided in an upper part of the inner tube at a fixed pitch along the axial length thereof. The nozzle openings are so devised as to have a larger diameter in regular succession from the inlet end to the opposite end of the inner tube; and a plurality of further nozzle openings are provided in the lower part of the outer tube at a fixed pitch along the axial length thereof and have a fixed diameter.

Material feed pipe for use in belt-system continuous vacuum drier

A fish meat powder retaining the functional characteristics of fresh fish meat is obtained by mincing and kneading fresh meat or frozen SURIMI (frozen ground fish meat), then subjecting the minced and kneaded meat to a colloid mill treatment to form a sol, foaming the resultant fish meat sol in a vacuum and drying the fish meat foam to form a thin sheet at low temperatures.

Method for the production of fish meat powder retaining functional

Vacuum type powder producing apparatus comprising an endless belt passing around a pair of vertically spaced rollers to provide a downward and upward runs, a vacuum chamber encircling only the downward run of the belt. Slurry of material is applied to the belt surfaces at the inlet end of the vacuum chamber and dried in the chamber under vacuum. Suitable belt cleaning device is provided outside the vacuum chamber.

Growing community of inventors

Oyama, Japan

Eitaro Kumazawa