Nippon Shokubai Co., Ltd.

Kobe University

Eita Ichige

Hisashi Semba

Masaharu Mukoyama

Akihiko Kondo

Masaharu Mukouyama

Keiji Nishida

Yukio Dobashi

Masahiro Nakanosho

Shigeaki Harayama

Shunichi Nakayama

Toshiaki Shijuku

The invention provides a method of modifying a targeted site of gram-positive bacterium of a double stranded DNA. The method includes contacting the double-stranded DNA with a complex of a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a given double stranded DNA and a nucleic acid base converting enzyme to convert, delete, or insert one or more nucleotides in the targeted site without cleaving at least one strand of the double stranded DNA in the targeted site, by introducing the nucleic acid encoding the complex into the gram-positive bacterium. The invention also provide a nucleic acid-modifying enzyme complex of a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a double stranded DNA of a gram-positive bacterium and a nucleic acid base converting enzyme bonded to each other, which complex is used for the method.

Method for converting genome sequence of gram-positive bacterium by specifically converting nucleic acid base of targeted DNA sequence, and molecular complex used in same

The present invention provides a means for producing butanol in butanol fermentation with high efficiency. The present invention relates to a genetically modified microorganism of, and a method for producing butanol using the microorganism.

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This invention relates to S-hydroxynitrile lyase having excellent tolerance to heat, organic solvents, and the like, which is obtained by modifying at least one amino acid in the helix D3, helix A, and &#x3b2;-sheet 2 domains in the amino acid sequence of wild-type S-hydroxynitrile lyase.

Modified S-hydroxynitrile lyase

A method for efficient production of an S-hydroxynitrile lyase by gene recombination usingis provided. A gene is formed by altering a codon in an S-hydroxyniitrylase gene originating in cassava (Crantz) without changing the amino acid sequence thereof till the frequency of codon usage wholly with an amino acid inreaches a level of not less than 5%. According to the method for the production of an S-hydroxynitrile lyase by the use of the gene, the S-hydroxynitrile lyase can be produced in a large amount with an unusually high yield.

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Tsukuba, Japan

Eita Ichige