Agency of Industrial Science and Technology

Agency of Industrial Science and Technology Ministry of International

Agency of Industrial Science & Technology, Ministry of International Trade & Industry

Einosuke Muraki

Shizu Fujishima

Fumiko Yaku

Ryutarou Tanaka

Masaru Miya

Naoko Yamano

A chitooligosaccharide residue is disclosed which is represented by the formula:

Chitooligosaccharide derivative

A partially N-acylated compound of chitooligosaccharide consisting essentially of 2 number of acyl glucosamine constituent units, m number of glucosamine constituent units and n number of acetyl glucosamine constituent units, wherein the ratios of l, m and n to the sum of l, m and n are in the respective ranges of 5 to 80%, 20 to 95%, and 0 to 50%, and having an OH radical attached at the 4 position or 1 position of the terminal unit of the composition.

Partially N-acylated compound of chitooligosaccharide, salt thereof, and

A process for producing deacetylase which comprises incubating a deacetylase-producing bacterium belonging to Vibrio cholerae in a IFO 15429 culture medium containing a carbon source, a nitrogen source, an inorganic salt and an inducer for producing the deacetylase and isolating the deacetylase from cells separated from said culture medium.

Process for producing deacetylase from Vibrio cholerea IFO 15429

A process for preparing a low-molecular weight chitosan which comprises adding chitosan to water containing a monobasic acid to prepare an intimate chitosan-water mixture and treating said mixture with cellulase.

Process for preparing chitosan oligosaccharides

A cellulosic material is saccharified by cellulases and the resulting saccharified solution is acidified. Subsequently chitosan and/or partially deacetylated chitin are dissolved therein and the obtained solution is alkalified. Thus the cellulases are absorbed by the chitosan and/or partially deacetylated chitin. Further a cellulosic material is saccharified by at least one cellulase originating from a fungus belonging to the genus Aspergillus and at least either chitosan and/or partially deacetylated chitin are added to the resulting saccharified solution to thereby adsorb the cellulase by the chitosan and/or partially deacetylated chitin.

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Osaka, Japan

Einosuke Muraki