Inscripta, Inc.

Eileen Spindler

Charles D Johnson

Tian Tian

Phillip Belgrader

Andrew Garst

Don Masquelier

Richard Fox

Bruce Chabansky

Michael Graige

Clint Davis

Amy Hiddessen

Miles Gander

Paul Hardenbol

Krishna Kannan

Isaac David Wagner

Julia Swavola

The present disclosure provides modules, instruments and methods to enrich for cells edited via nucleic acid-guided nuclease editing of live cells.

Detection of nuclease edited sequences in automated modules and instruments

The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells. The compositions and methods presented herein in combination lead to a phenomenon of 'edit or die.' Although less cells survive plating and editing, a large percentage of cells that do survive are multiple editors. In one experiment it was found that if a cell survives transformation, plating, and editing, 75% of the surviving cells are multiple editors; that is, 75% of the surviving cells were simultaneously edited with edits at two or more different locations within the bacterial genome.

Methods for increasing observed editing in bacteria

The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules&#x2014;including high throughput modules&#x2014;for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.

The present disclosure provides automated multi-module instrumentation and automated methods for performing recursive editing of live cells with curing of editing vectors from prior rounds of editing.

Curing for recursive nucleic acid-guided cell editing

The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells.

The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods.

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Boulder, CO, United States of America

Eileen Spindler