Mayo Foundation for Medical Education and Research

Eileen M McGowan

Michael L Hutton

Todd Eliot Golde

Jada M Lewis

Pritam Das

The invention provides constructs that feature a nucleic acid molecule encoding an amino terminal region of a BRI polypeptide. A construct of the invention can further include a multiple cloning site joined to the 3' end of the BRI nucleic acid molecule or a nucleic acid molecule encoding a heterologous polypeptide operably linked to the BRI nucleic acid molecule. The invention further provides methods of directing a heterologous polypeptide through the secretory pathway in a cell. Such methods utilize a construct of the invention that additionally contains a promoter that directs expression of the BRI and heterologous nucleic acid molecules. The construct is introduced into a cell and, following expression, the fusion protein is directed through the secretory pathway of the cell. In addition, a construct of the invention can be introduced into an animal to make the animal transgenic for the heterologous polypeptide.

BRI constructs and methods of using

Isolated nucleic acid molecules that include a tau gene sequence are described. The tau gene sequences have a mutation linked to a Tau pathology. Transgenic non-human mammals that develop a Tau pathology also are described.

Growing community of inventors

Jacksonville, FL, United States of America

Eileen M McGowan