Other

Eileen Adamson

Daniel Mercola

Ian De Belle

Dan Mercola

The present invention provides methods and compositions for isolating a nucleic acid molecule fragment comprising at least a portion of a gene, comprising: stimulating at least one cell or at least one nucleus with radiation; cross-linking at least one transcription factor to a nucleic acid molecule in said at least one cell or at least one nucleus with formaldehyde, forming at least one transcription factor-nucleic acid molecule complex; fragmenting said nucleic acid molecule to form at least one transcription factor-nucleic acid molecule fragment complex; and isolating the nucleic acid molecule fragment from said at least one transcription factor-nucleic acid molecule fragment complex to form at least one isolated nucleic acid molecule fragment; wherein said at least one isolated nucleic acid molecule fragment comprises at least a portion of the first exon of a gene whose expression is modulated by said transcription factor; further wherein said at least one isolated nucleic acid molecule fragment comprises at least one transcription factor binding site that is operably linked or in close proximity to said first exon of a gene.

Isolation and identification of control sequences and genes modulated by transcription factors

The present invention provides methods for the identification of nucleic acid molecules corresponding to genes regulated by a transcription factor comprising: cross-linking a transcription factor to a nucleic acid molecule in a cell forming a transcription factor-nucleic acid molecule complex; fragmenting the nucleic acid molecule to form a transcription factor-nucleic acid molecule fragment complex; isolating the nucleic acid molecule fragment; combining the isolated nucleic acid molecule fragment with a cDNA library of sequences known to be complementary to previously identified nucleic acid molecules, or a cDNA obtained by reverse transcription of a population of RNA molecules, to form a mixture comprising an isolated nucleic acid molecule fragment-cDNA complex; amplifying the cDNA in the isolated nucleic acid fragment-cDNA complex using the nucleic acid molecule fragment as a primer; and identifying the amplified cDNA molecule by sequencing the cDNA molecule and comparing to known sequences or hybridization to known sequences.

This invention is directed to methods for inhibiting the growth in vitro or in an individual of a tumor cell induced by the mitogenic activity of PDGF by transfecting the cell with an expression vector having an expression control sequence operatively linked to a nucleic acid sequence encoding a mammalian EGR, a nucleic acid sequence encoding a fragment of a mammalian EGR comprising the three zinc fingers or a nucleic acid sequence that hybridizes to any of the foregoing nucleic acid sequences under standard hybridization conditions and that encodes a polypeptide having the cell growth-inhibiting activity of Egr-1.

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Rancho Santa Fe, CA, United States of America

Eileen Adamson