Sloan-kettering Institute for Cancer Research

Eileen A Friedman

Lloyd J Old

Kenneth O Lloyd

Herbert F Oettgen

Carlos Cordon-Cardo

Myron R Melamed

Junichi Sakamoto

Connie L Finstad

Martin Lipkin

Warren E Enker

The invention concerns a method for the routine primary culture of human colonic epithelial cells within a monolayer. 12-0-tetradecanoylphorbol-13-acetate (TPA) enhanced DNA synthesis an average of 8 fold when assayed by [.sup.3 h]dThd labeling indices (L.I.) in colonic epithelial cells from familial polyposis patients and in tubular adenomas. No such stimulation by TPA was seen in cells from high-risk patients without familial polyposis, or in cells from low-risk subjects.

Method for growth in tissue culture of normal colonic epithelial cells

A panel of monoclonal antibodies produced from human gastrointestinal tumors as immunogen is used to diagnose the presence of colon cancer. The antibody panel subsets the human gastrointestinal tract in its reactivity vis-a-vis esophagus, stomach, small intestine and colon. The panel is useful as a diagnostic probe for cancer.

Monoclonal antibodies to human gastrointestinal cancers and hybridoma

Growing community of inventors

New York, NY, United States of America

Eileen A Friedman