Idemitsu Kosan Company, Limited

National Institute of Advanced Industrial Science and Technology

National Center for Global Health and Medicine

Hokusan Co. Ltd

Eiji Takita

Kazuyoshi Koike

Takeshi Matsui

Koichi Kaneda

Kazutoshi Sawada

Akira Ito

Takeshi Matsumura

Noriko Tabayashi

Takashi Hamabata

Toshio Sato

By linking a peptide tag having the amino acid sequence X(PY)PZ to a protein of interest, and then expressing the protein, the protein of interest is efficiently accumulated in a soluble fraction, where P is proline; X is an amino acid sequence composed of 0 to 5 amino acids independently selected from R, G, S, K, T, L, N, Q, and M; Y is an amino acid sequence composed of 1 to 4 amino acids independently selected from R, G, K, T, L, N, Q, and M; q is an integer 1 to 10; and Z is an amino acid sequence composed of 0 to 10 amino acids independently selected from R, G, S, K, T, L, N, Q, and M; where the amino acid sequence of the peptide tag contains three or more Qs, Ms, Ls, Ns, and/or Ts in total.

Soluble expression of protein using peptide tag

A peptide comprising the sequence shown below is added as a peptide tag to a useful protein, followed by allowing its expression.X(PY)PZ In this formula, X, Y, and Z each represent an amino acid residue independently selected from the group consisting of R, G, S, K, T, L, N, Q, and H, with the proviso that at least one Y represents K, L, N, Q, H, or R. m represents an integer of 0 to 5; n represents 1, 2, or 3; q represents an integer of 1 to 10; and r represents an integer of 0 to 10.

Peptide tag and tagged protein including same

The present invention provides an agent for controlling colibacillosis including a fusion protein comprising a subunit of Shiga toxin and a subunit ofheat-labile toxin.

Vaccine against colibacillosis

The present invention provides an agent for controllingdiarrhea comprising a Shiga toxin as an active ingredient.

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Sodegaura, Japan

Eiji Takita