Other

Eiji Ohara

Yasuhiro Furuichi

Akira Shimamoto

Masanori Watahiki

Hiroko Funaki

Yuko Shibata

Shoji Otsuki

Kenjiro Kuroishi

cDNA including the 5&prime;-terminal sequence of full-length mRNA with a cap structure is synthesized from a mRNA sample containing the full-length mRNA with the cap structure and non-full-length mRNA without any cap structure in mixture. At the first step, the phosphate group at 5&prime;-terminus of the non-full-length mRNA in the mRNA sample is removed. At the second step, the cap structure at the 5&prime;-terminus of the full-length mRNA in the mRNA sample is removed. At the third step, an oligoribonucleotide is ligated to the phosphate group at 5&prime;-terminus of mRNA generated through the first and second steps. At the fourth step, mRNA with the oligoribonucleotide ligated at the 5&prime;-terminus thereof at the third step is subjected to a reverse transcriptase process using a short-chain oligonucleotide capable of being annealed to an intermediate sequence within the mRNA as primer, to synthesize a first-strand cDNA.

Method for synthesizing cDNA from mRNA sample

This invention provides an intertwisting means including drum rotatable about the axis thereof to feed linear objects wound thereon through arm members suspended over the periphery of the drum in rotatable relation with the axis thereof so as to guide the fed objects into an inner hollow portion of the drums by means of guide rollers. The invention further involves the construction formed with cap means for intertwisting the guided objects at an equal angle of torsion thereby to form a continuity of thick, strong rope-like object and a means for automatically winding said continuity thereon.

Twisting machine

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Toyama, Japan

Eiji Ohara