Kyoto University

Osaka University

Nipro Corporation

Eihachiro Kawase

Norio Nakatsuji

Hirofumi Suemori

Shinya Yamanaka

Kiyotoshi Sekiguchi

Motonari Uesugi

Masato Nakagawa

Atsushi Taguchi

Kazumitsu Ueda

Takamichi Miyazaki

Ryo Tomii

Sugiko Futaki

Yukimasa Taniguchi

Yuto Fujibayashi

Maria Hayashi

Nao Hirata

Asako Murata

Young-Tae Chang

Tsuneo Takahashi

Kaori Yamauchi

A biological sample or drug stored inside a frozen storage container is not susceptible to contamination. The frozen storage container includes a container body with an internal space, and a partition wall which divides the internal space at least into a first space and a second space. In the container body, the first space is liquid-tightly sealed, the second space has an opening which is in communication with the outside, and the second space is liquid-tightly sealable by welding the container body. A frozen storage container system includes the frozen storage container; and a needle member which includes a needle tip portion capable of being inserted into the internal space of the container body and capable of piercing the partition wall, and has a flow path which is opened at the needle tip portion and is opened at a base end portion opposite to the needle tip portion.

Frozen storage container and frozen storage container system

A method for sorting pluripotent cells using a compound which is eliminated from the pluripotent cells through the MDR1 transporter.

Method for sorting of pluripotent cells

The present invention provides a culture substrate which enables maintenance culture of human pluripotent stem cells in a pluripotent state under a feeder-free culture environment, and a culture method of human pluripotent stem cells using the culture substrate. By seeding human pluripotent stem cells dissociated into single cells at a cell density of 4&#xd7;10to 10&#xd7;10cells/cmonto a culture substrate coated with human laminin &#x3b1;5&#x3b2;1&#x3b3;1 E8 fragment or human laminin &#x3b1;3&#x3b2;3&#x3b3;2 E8 fragment preferably at a concentration of 0.5 to 25 &#x3bc;g/cm, the human pluripotent stem cells can be rapidly expanded in a pluripotent state.

Culture substrate for human pluripotent stem cells and use thereof

Growing community of inventors

Kyoto, Japan

Eihachiro Kawase