Symrise Ag

Egon Gross

Jakob Peter Ley

Gerhard Krammer

Michael Backes

Günter Kindel

Susanne Paetz

Uwe Bornscheuer

Thomas Riess

Katharina Reichelt

Kathrin Langer

Thomas Scheper

Johannes Kiefl

Jenny Weissbrodt

Maren Thomsen

Holger Zorn

Jens-Michael Hilmer

Torsten Geissler

Fabia Hentschel

Katrin Geissler

Jens Michael Hilmer

Marco A Fraatz

Johanna Rost

Michael Goldmann

Winfried Hinrichs

Mechthild Gall

Christin Peters

Patrick Jonczyk

Sascha Beutel

Tom Somers

Stephan Drusch

Morine Verwohlt

Rebecca Wiebusch

Sandra Heinert

Marco Fraatz

Dariah Lutsch

A method for producing branched aldehydes is proposed, the method comprising the following steps:

Method for producing branched aldehydes

The invention relates to a method for producing flavone glycoside dihydrochalcones, having the following steps: (a) providing a transgenic microorganism containing (i) a first nucleic acid portion (A) containing a gene which codes for a bacterial chalcone isomerase and (ii) a second nucleic acid portion (B) containing a gene which codes for a bacterial enoate reductase, (b) adding one or more flavone glycosides to the transgenic microorganism under conditions which allow the simultaneous isomerization and reduction of the flavone glycoside into the flavone glycoside dihydrochalcone, and optionally (d) isolating and purifying the final product, wherein the nucleic acid portion (A) (1) is a nucleotide sequence according to SEQ ID NO:1, in which the nucleic acid portion (A') according to SEQ ID NO:3 has been cut out, or (2) is an amino acid sequence according to SEQ ID NO:2, in which the amino acid portion (A&#x2032;) according to SEQ ID NO:4 has been cut out.

Method for the biotechnological production of flavone glycoside dihydrochalcones

A method for production of a dihydrochalcone, especially of phloretin, using a transgenic microorganism, containing a nucleic acid section (a), comprising or consisting of a gene coding for a bacterial chalcone isomerase, and/or a nucleic acid section (a'), comprising or consisting of a gene coding for a plant chalcone isomerase, and a nucleic acid section (b), comprising or consisting of a gene coding for a bacterial enoate reductase, corresponding transgenic microorganisms, containing a nucleic acid section (a), comprising or consisting of a gene coding for a bacterial chalcone isomerase, and/or a nucleic acid section (a&#x2032;), comprising or consisting of a gene coding for a plant chalcone isomerase, and/or a nucleic acid section (b), comprising or consisting of a gene coding for a bacterial enoate reductase, and host cells, containing one or more identical or different such vectors.

Method for biotechnological production of dihydrochalcones

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Holzminden, Germany

Egon Gross