Hoechst Japan Limited

Behringwerke Aktiengesellschaft

Aventis Behring Gmbh

Zlb Behring Gmbh

Behringwerde Aktiengesellschaft

Egon Amann

Ulrich Grundmann

Sunao Takeshita

Karl-Josef Abel

Gerd Zettlmeissl

Shinji Kawai

Makoto Okazaki

Stefan Knapp

Yoko Otawara-Hamamoto

Reiko Kikuno

Atsushi Tsujimura

Toshimi Ito

Kenichi Tezuka

Gerd Zettlmeibl

The cDNA which codes for factor XIIIa has been isolated using a cDNA bank from human placenta and probes constructed on the basis of the amino acid sequence of factor XIIIa peptide fragments. It is possible with this cDNA not only to obtain factor XIIIa by gene manipulation in high purity but also to prepare diagnostic aids which permit the analysis of genetic factor XIIIa defects. Furthermore, it is possible on the basis of the amino acid sequence to prepare antibodies which are suitable for diagnostic aids and antibody columns.

Preparation of factor XIIIa by gene manipulation

Preparation of DNA encoding factor XIIIA

A bone-related protein named OSF-4 which is obtained from bone tissue of a mammal including mouse or human, and a process for its production. This protein is a novel naturally occurring mammal protein of the cadherin family. OSF-4 acts as an adhesion molecule or a growth factor which takes part in the process of osteogenesis at the site of bone induction. OSF-4 can be used as an agent for treating bone metabolic diseases, and its high organ specificity for bones enables its use as a diagnostic reagent for bone metabolic diseases.

Bone-related cadherin-like protein and process for its production

The present invention provides the sequence of the novel bone-related protein OSF-2 which can be obtained from mammalian bone tissues. The invention further provides a method of production of OSF-2 by recombinant techniques.

Protein with bone formation ability and process for its production

A bone-related protein named OSF-8 which is obtained from bone tissue of a mammal including mouse or human, and a process for its production. This protein is a novel naturally occurring mammal protein which belongs to a group of sulfatases. OSF-8 degrades the sulfate groups of the sugar chains of proteoglycan, which makes up the cartilage matrix, during the replacement of cartilage into bone tissue at the stage of osteogenesis. OSF-8 can be used as an agent for treating bone metabolic diseases, and its high organ specificity for bones enables its use as a diagnostic reagent for bone metabolic diseases.

Bone-related sulfatase-like protein and process for its production

The invention relates to a nucleic acid encoding a signal peptide from Bordetella pertussis, a recombinant molecule comprising the signal peptide, and processes for optimizing protein expression in Gram-negative bacteria employing the nucleic acid or signal peptide.

Nucleic acid encoding a signal peptide, a recombinant molecule

Bone-associated proteins derived from bone of mammals, including mice and human beings, named OSF-6. The protein is the novel natural type mammal protein, which can play an important role in bone formation, and belongs to a group of transcription control factors. OSF-6 can be used as a therapeutic agent for bone metabolic diseases, and also as a diagnostic agent for bone metabolic diseases, since it may demonstrate a high organ specificity to bone.

Bone-associated transcription control factor-like protein

A bone-related carboxypeptidase-like protein named OSF-5 which is obtained from bone tissue of a mammal including mouse or human, and a process for its production. This protein is a novel naturally occurring mammal protein which belongs to a group of carboxypeptidase molecules. OSF-5 acts as an adhesion molecule or a growth factor which takes part in the process of osteogenesis at the site of bone induction. OSF-5 can be used as an agent for treating bone metabolic diseases, and its high organ specificity for bones enables its use as a diagnostic reagent for bone metabolic diseases.

Bone-related carboxypeptidase-like protein and process for its production

The protein PP4-X, whose amino acid sequence and the DNA sequence coding for this have been determined, has anticoagulative properties and can be prepared by genetic manipulation. PP4-X is used for therapeutic and diagnostic purposes.

DNA encoding anticoagulative protein PP4-X, and its preparation and use

Anticoagulative protein PP4-X

The invention relates to a new signal peptide from Bordetella pertussis with the amino acid sequence M K K W F V A A G I G A G L L M L S S A A and to particularly suitable expression vectors with whose aid such signal sequences can be found and/or evaluated.

Growing community of inventors

Marburg, Germany

Egon Amann