Ambergen, Inc.

Helicos Biosciences Corporation

Institute for Environmental Health, Inc.

Phage Diagnostics, Inc.

Edyta Krzymanska-Olejnik

Jerzy Olejnik

Kenneth J Rothschild

Jayson Linn Bowers

Atanu Roy

Subramanian Marappan

Suhaib Siddiqi

Michael Koeris

Daniel Robert Brownell

Xiaopeng Bai

Robert Patrick Shivers

Sergey Mamaev

Judith Mitchell

Michael Cappillino

J William Efcavitch

Philip Richard Buzby

Mirna Jarosz

Timothy Kuan Ta Lu

Julie Thomason

Composition and methods for the detection of one or more target microbe(s) are provided. Compositions of the disclosure include at least one recombinant phage capable of infecting a target microbe, said phage comprising at least a capsid protein sequence, a ribosome binding site, and a codon-optimized marker. Compositions of the disclosure may further include an aqueous solution that enhances the ability to detect marker expression upon phage infection of the target microbe. In some embodiments the target microbe include is

Codon optimized recombinant phage and methods of using same

Methods of detecting target bacteria are provided. In some embodiments the methods comprise exposing the sample to a phage capable of infecting a set of target bacteria and comprising a heterologous nucleic acid sequence encoding a marker. In some embodiments the target bacteria comprise. In some embodiments the target bacteria are all. Recombinantphage comprising a heterologous nucleic acid sequence encoding a marker are also provided as are useful combinations of such phage and articles of manufacture comprising such phage, among other things.

Phage-based bacterial detection assay

The invention provides for nucleotide analogs and methods of using the same, e.g., for sequencing nucleic acids.

Nucleotide analogs

Labelled nucleotides and polynucleotides useful in the sequencing of nucleic acids are described. Methods of preparing photocleavable marker nucleotides and photocleavable marker-polynucleotide conjugates are described. Such photocleavable markere nucleotides can be incorporated into nucleic acid so as to create photocleavable marker-polynucleotide conjugates.

Nucleotide compositions comprising photocleavable markers and methods of preparation thereof

The present invention relates to methods for the preparation of chemically aminoacylated tRNAs for the purpose of introduction of markers into nascent proteins. The present invention also relates to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system utilizing chemically aminoacylated tRNAs. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system.

Methods for the preparation of chemically misaminoacylated tRNA via protective groups

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Brookline, MA, United States of America

Edyta Krzymanska-Olejnik