Illumina Corporation

Illumina Cambridge Limited

Illumina Singapore Pte. Ltd.

Edwin Li

Sean M Ramirez

Alexandre Richez

Ludovic Vincent

Wei Wei

David Louis Heiner

John M Beierle

James M Tsay

Sang Ryul Park

Kevan Samiee

Peyton Shieh

Scott William Bailey

Tyler Jamison Dill

Anmiv Prabhu

Robert Yang

Naiqian Zhan

Yuxiang Huang

Samantha Schmitt

Sunmin Ahn

Valerie Uzzell

Kristina Munoz

Leonid Malevanchik

Allen E Eckhardt

Hai Quang Tran

Lewis J Kraft

Brian Douglas Mather

Rigo Pantoja

Xavier Von Hatten

Hong Xie

Petr Capek

Julia Morrison

Gianluca Andrea Artioli

Maxwell Zimmerley

Hayden Black

Innsu Daniel Kim

Krystal Sly

Sojeong Moon

Ryan Sanford

A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.

Flow cell package and method for making the same

An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.

Protective surface coatings for flow cells

Embodiments of present application are directed to microfluidic devices and particularly digital micro-plastic fluidic devices that are specifically designed to prevent sample contamination during sample processing, methods of manufacturing the same, and methods to improve sample analysis process by preventing sample contamination.

Cationic polymers and method of surface application

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Los Angeles, CA, United States of America

Edwin Li