Intervet Inc.

Intervet International B.v.

Edwin Kets

Paul Vermeij

Martin Piest

Christian Theodoor Gerardus Smits

Chris Dirks

Henriette Adriaanse

Nöel Dautzenberg

Marian Anna Catharina Jozefina Heijnen

Christian Theodoor Smits

The present invention relates to a liquid composition of live Mollicutes bacteria and a stabiliser, whereby the stabiliser is a natural deep-eutectic solvent (NADES). In this liquid composition the live Mollicutes are stabilised without need for freezing or freeze-drying. This allows various advantageous uses in diagnostics and medicine, specifically as a liquid vaccine for use against infection or disease caused by Mollicutes bacteria, for human- or non-human animals.

Stabilisation of live mollicutes bacteria in a liquid composition

The present invention describes a liquid vaccine composition of a live enveloped virus and a pharmaceutically acceptable carrier, whereby the carrier is a natural deep-eutectic solvent (NADES), and the vaccine has a water activity of less than about 0.8. The NADES provides a stabilisation of the sensitive virus for prolonged time and at ambient temperature. In general, the liquid vaccine compositions according to the invention, in different compositions for the various enveloped viruses, show remarkable capabilities of stabilisation. This overcomes the need for lyophilisation, a great economic benefit. Also the liquid nature of the vaccines facilitates administration to human or animal targets.

Liquid vaccines of live enveloped viruses

The antigenic mass ofcultures can be significantly increased, independent from any increase in biomass, by supplementingcultures with a specific type of fatty acid: a polyunsaturated C18 fatty acid. This provides advantages in the productionantigens. Also this enables the production of improved Leptospirosis vaccines, that are safer and more effective.

Leptospira with increased antigenic mass

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Heilig Landstichting, Netherlands

Edwin Kets