Sartorius Ag

Appligene-Oncor S.a.

Edwige Bonfils

Daniel Dupret

Dietmar Nussbaumer

Wolfgang Demmer

Khuong To Vinh

Ulysse Asseline

Hong Khanh Nguyen

Thang Thuong Nguyen

Maurice Durand

Thuong Nguyen

Jean-Claude Maurizot

The invention discloses a method for producing a hybridization complex whose stability is substantially independent of the base composition of two hybridized nucleic acid molecules, consisting in contacting a first nucleic acid molecule with a second nucleic acid molecule under conditions enabling the formation of a specific hybridization complex. The invention is characterized in that at least one of the four types of bases contained in the sequence of the first and/or of the second nucleic acid molecule is a modified base having pairing properties close to those of the natural base it replaces, such that at least one of the two types of the base pair contained in the composition of the complex comprises a modified base and in that the totality of the base pairs contained in the composition of the said complex has a like stability.

Method for producing hybridization complexes whose stability is substantially independent of the base composition of two hybridized nucleic acid molecules

The abstract discloses a method for preparing supports for the assay of chemical or biological substances, consisting in preparing N different supports, each being homogeneous for a type of reaction, then in mutually combining, according to a predetermined arrangement, all or part of the said homogenous N supports to constitute assay supports having each an ordered set of reaction zones of N different types or less. The invention also concerns the assay supports prepared by this method and their use in processes of immunological hybridisation assays.

Method for preparing assay supports having an ordered set of reaction zones

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Edwige Bonfils