The General Hospital Corporation

The United States of America, As Represented by the Secretary, Department of Health and Human Services

Oxford University Innovation Limited

International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b)

International Centre for Diarrhoeal Disease Research, Bangladesh

Edward T Ryan

Stephen B Calderwood

Firdausi Qadri

Pavol Kovac

Dwight Christopher Peterson

Peng Xu

Richelle Charles

Stephen Baker

Willie Frank Vann

Provided are assays, kits and compositions for testing subjects, particularly asymptomatic subjects, to ascertain whether or not they are carriers ofserotype. Methods for detecting the presence ofserotypeindicating molecules by a variety of methods such as immunoassays and mass spectrometry also are provided.

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The disclosure provides directly conjugated polysaccharide vaccine molecules and methods related thereto.

Conjugating amines

Compositions and methods for secreting large heterologous polypeptides are described.

Disclosed is a V. cholerae cell containing a DNA encoding a fusion polypeptide that includes a heterologous antigenic polypeptide sequence such as a portion of C. difficile toxin A. The fusion polypeptide also includes one of the following: (i) an E. coli hemolysin A subunit (wich is coexpressed with sequences encoding hemolysin B and D subunits); (ii) a secretion signal sequence and cholera toxin A2 subunit (which is coexpressed with cholera toxin B subunit); or (iii) cholera toxin B subunit.

Heterologous antigens in live cell V. cholerae strains

Growing community of inventors

Wellesley, MA, United States of America

Edward T Ryan