Roche Molecular Systems, Inc.

Other

Edward Soh Smith

Thomas W Myers

David H Gelfand

Keith Bauer

Shawn Lee Suko

Ellen H Fiss

Dirk Zimmermann

Meike Eickhoff

Nancy J Schoenbrunner

Eberhard Russmann

Christopher D Newhouse

Andreas Woelfelschneider

Sean F Boyle

Tomas R Martinez

Stephen Gordon Will

Russell G Higuchi

Alice M Wang

Susanne Stoffel

Nicolas Newton

Heike Wilts

John Niemiec

Niclas Hitziger

Carita Maria Elfstrom

Nancy Jeneane Schönbrunner

Christopher Newhouse

Anke Mussmann

Olga Budker

The present invention relates to the sample preparation of nucleic acids for diagnostic purposes. More precisely, the invention provides a process for simultaneously isolating at least a first and a second target nucleic acid from a plurality of different types of fluid samples and optionally amplifying said isolated nucleic acids in a simultaneous manner.

Generic sample preparation

The present invention provides stable dried reaction mixtures, methods for their preparation, methods for their use, and kits comprising them. The stable dried reaction mixtures are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).

Reaction mixtures

Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

DNA polymerases and related methods

The present invention provides a method for the amplification of at least a first and a second target nucleic acid that may be present in a fluid sample. The invention further provides a kit and an analytical system for carrying out said amplification.

Generic PCR

Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. The mutant polymerases overcome the inhibitory effects by an intercalating dye. Therefore, the mutant polymerases are useful in a variety of disclosed methods in combination with an intercalating dye. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

The present invention relates to improved reverse transcription methods using a modified thermostable DNA polymerases, particularly in a magnesium ion buffer. These methods are particularly useful in combined reverse-transcription/amplification reactions.

High temperature reverse transcription using mutant DNA polymerases

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San Francisco, CA, United States of America

Edward Soh Smith