European Molecular Biology Laboratory

Embl

Edward Lemke

Carsten Schultz

Ivana Nikic

Tilman Plass

Christine Köhler

Sigrid Milles

Iker Valle Aramburu

Christine Koehler

Jan-Erik Hoffmann

Gemma Estrada Girona

Tillmann Plass

Jan-Erik Hoffman

The invention relates to archaeal pyrrolysyl tRNA synthetases lacking a nuclear localization signal and/or comprising a nuclear export signal. The invention also relates to polynucleotides encoding said pyrrolysyl tRNA synthetases, eukaryotic cells comprising said polynucleotide and tRNA acylated by the pyrrolysyl tRNA synthetase or a polynucleotide encoding such tRNA, methods utilizing said cells for preparing polypeptides comprising unnatural amino acid residues, and kits useful in said methods.

Archaeal pyrrolysyl tRNA synthetases for orthogonal use

The present invention relates to compounds of formula: and methods for linking tetrazines with dienophiles to establish at least two linkages by sequentially performing at least two cycloaddition reactions. The methods in particular allow establishing multi-labeling strategies. In particular, the invention relates to methods for forming linkages by cycloaddition reactions, wherein the method comprises reacting a first alkyl-substituted tetrazine with a first dienophile comprising a trans-cyclooctenyl group followed by reacting a second tetrazine with a second dienophile comprising a cyclooctynyl group, wherein the reaction of the first tetrazine with the first dienophile proceeds in the presence of the second dienophile.

Trans-cyclooctene amino and hydroxy acids and their use in multiple cycloaddition reactions for labeling of molecules

The present invention relates to unnatural amino acids comprising a cyclooctynyl or trans-cyclooctenyl analog group and having formula (I) or an acid or base addition salt thereof. The invention also relates to the use of said unnatural amino acids, kits and processes for preparation of polypeptides that comprise one or more than one cyclooctynyl or trans-cyclooctenyl analog group. These polypeptides can be covalently modified by in vitro or in vivo reaction with compounds comprising an azide, nitrile oxide, nitrone, diazocarbonyl or 1,2,4,5-tetrazine group. 

Unnatural amino acids comprising a cyclooctynyl or trans-cyclooctenyl analog group and uses thereof

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Mannheim, Germany

Edward Lemke