Cetus Corporation

Edward L Sheldon, Iii

Henry Rapoport

Kary B Mullis

Corey H Levenson

Robert M Watson

Richard M Snead

Nucleic acids may be labeled by complexing the alkylating moiety of a labeling reagent into a single-stranded nucleic acid to form a complex and activating the complex to cause covalent bonding between the reagent and the nucleic acid. Preferably, the labeled nucleic acid is a single-stranded hybridization probe for detecting nucleic acid sequences capable of hybridizing with a hybridizing region of the nucleic acid. Also preferably the label moiety is non-radioactive. The labeling reagent is of the formula:

Process for labeling single-stranded nucleic acids and hybridizaiton

Nucleic acids may be labeled by intercalating the alkylating intercalation moiety of a labeling reagent into a partially double-stranded nucleic acid to form a complex and activating the complex to cause covalent bonding between the reagent and the nucleic acid. Preferably, the labeled nucleic acid is a hybridization probe for detecting nucleic acid sequences capable of hybridizing with a hybridizing region of the nucleic acid. Also preferably the label moiety is non-radioactive. The labeling reagent is of the formula:

Process for labeling nucleic acids and hybridization probes

Process for labeling nucleic acids using psoralen derivatives

Growing community of inventors

Oakland, CA, United States of America

Edward L Sheldon, Iii