Arizona State University

Chevron U.s.a. Inc.

Edward Kavazanjian

Nasser Hamdan

Hamed Khodadadi Tirkolaei

Abdullah Almajed

Thomas Hoelen

Rolf Ulrich Halden

Ximin He

Zhi Zhao

Alizee M Jenck

Neda Javadi

Disclosed are methods of extracting an enzyme, comprising soaking a seed in an aqueous solution, homogenizing the seed to produce a homogenized suspension, and filtering the homogenized suspension to produce a crude extract, wherein the crude extract comprises urease and non-urease proteins.

Enzyme extraction methods

The present disclosure provides a method of biocementation comprising contacting a granular, cohesionless soil with a solution, wherein the solution comprises urea, urease, a source of calcium ions, and a source of non-urease proteins, wherein the urea, urease, source of calcium ions, and source of non-urease proteins are provided in effective amounts suitable to cause crystallization of calcium carbonate.

Biocementation systems and methods

The present invention provides methods for mineral precipitation and/or cementation of permeable or fractured non-porous materials using isolated urease.

Cementation methods

A soil stabilization method of forming an admixture of at least one hydrogel precursor and urease enzyme, and contacting at least a portion of the admixture with soil, and forming a hydrogel network in-situ within at least a portion of the soil, wherein at least a portion of the hydrogel network includes in-situ precipitated calcium carbonate. In some embodiments, at least a portion of the hydrogel network is formed in-situ by polymerizing the at least one hydrogel precursor in the presence of the urease enzyme.

Hydrogel biocement systems and methods

The present invention provides methods for mineral precipitation of porous particulate starting materials using isolated urease.

Mineral precipitation methods

A method for installing an in situ, low-permeability temporary horizontal barrier at depth for vertical containment of a soil mass including installing a low permeability vertical barrier having walls located to contain a predetermined soil volume, the vertical barrier having a horizontal surface area in a plane perpendicular to its walls; and injecting low permeability reagents capable of forming a temporary horizontal barrier in a pattern suitable for creating a horizontal subsurface barrier joined to all of the vertical barrier walls to form an in situ barrier.

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Tempe, AZ, United States of America

Edward Kavazanjian