Ge Healthcare Bio-sciences Corp.

Edward John Moler

Alberto Santamaria-Pang

Brion Daryl Sarachan

Yousef Ahmed Al-Kofahi

Qing Li

Raghav Krishna Padmanabhan

The disclosed subject matter relates to an automated determination of cell-by-cell segmentation quality of a tissue specimen sample. A training set of cells is examined by an expert to determine which cells that include 'good' segmentation and which cells include 'poor' segmentation. A training model is build based on the image data of the cells in the training set. Image data from cells in a test specimen is obtained and that image data is compared to the training model to determine on a cell-by-cell basis which cells in the test specimen include &#x201c;good&#x201d; segmentation and which cells include &#x201c;poor&#x201d; segmentation. The accumulated data on the cells analyzed in the test specimen can be utilized to determine an overall segmentation quality score for the area of the test specimen in which the individual cells are located in the test specimen.

Automated cell segmentation quality control

The disclosed subject matter relates to an automated determination the stain quality and segmentation quality of a tissue sample. By way of example, separate image data is acquired of an unstained form of a biological specimen, the biological specimen stained with a nuclei marker and the biological specimen stained with a segmentation marker. A correlation map (Cr) from the separate image data and a ridgeness map (Pr) from the image data of the biological specimen stained with a segmentation marker are each determined. A staining quality score and segmentation quality score are then determined from the correlation map (Cr) and the ridgeness map (Pr).

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Aliso Viejo, CA, United States of America

Edward John Moler