Monsanto Technology LLC

Wisconsin Alumni Research Foundation

Edward James Williams

Brian J Martinell

Xudong Ye

Yuechun Wan

Michael W Petersen

David A Somers

Kevin Lee Deppermann

Jerald D Heise

Cindy L Ludwig

Angela Ranae Koestel

Beth Jo Calabotta

Erik D Dersch

Shawn Michael Kaeppler

Heidi Flewelling Kaeppler

Michael William Petersen

Dennis E McCabe

Yong Huang

Junjiang Shen

Lori S Julson

Carol A Emler

Jyoti R Rout

Whitney R Adams, Jr

Frank McFarland

Robert Wayne Harnish

Ray Collier

Anatoly Rivlin

Yurong Chen

Amy Jo Miyamoto

Ashok Shrawat

Nathaniel Schleif

Anna Mary O'KEEFE

David Alan Somers

Whitney R Adams

The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

Methods for plant transformation using spectinomycin selection

Disclosed herein are methods for sterile extraction, drying, storage, and transformation of immature and mature maize and monocot embryos. The present disclosure also describes a dried, storable, freezable value added maize and monocot explants.

Efficient monocot embryo extraction and preservation methods and novel uses thereof

Disclosed herein are methods for the production ofmeristem explants from dry seeds. Also described are methods of transforming and gene editing using themeristem explants disclosed herein.

Methods of gene editing and transforming

The present invention provides methods for the production of viable explants from mature corn seeds, wherein the explant comprises the apical portion of the embryo axis of the corn seed. The present invention also relates to methods for producing such explants and for transforming the explants with a heterologous DNA.

Methods for transforming corn explants

The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

Preparation and use of plant embryo explants for transformation

Apparatus for the preparation and use of plant embryo explants for transformation

Methods of gene editing and transforming cannabis

The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture.

Methods for improving competency of plant cells

The present disclosure provides methods for the stable transformation of meristem explants from cowpeas () and dry beans ().

Agrobacterium-mediated and particle bombardment transformation method for cowpea and dry bean meristem explants

Disclosed herein are methods for the production of value added explants from the seeds of dicotyledonous cultivars of interest. Also described are methods of transformation using the value added explants produced by the methods disclosed herein.

Efficient plant transformation method

The present invention provides methods for improving competency of plant cells, such as corn plant immature embryo for bacterial-mediated transformation comprising contacting the plant cells, such as corn plant immature embryo with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells, such as corn plant immature embryo for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells, such as corn plant immature embryo in various storage conditions, thus improving their competency for transformation and tissue culture.

Methods for improving competency of corn plant immature embryo

The invention relates to methods for Rhizobia-mediated genetic transformation of plant cells, including soybean, canola, corn, and cotton cells. These include both VirD2-dependent and VirD2-independent methods. Bacterial species utilized include strains ofsp.,sp., andsp. Vectors for use in such transformation are also disclosed.

Use of non-bacterial species for plant transformation

Growing community of inventors

Madison, WI, United States of America

Edward James Williams