Monsanto Technology LLC

Edward James Cargill

Ty Jason Barten

Dennis Hung Yang

Jose Rafael Gomez

Manuel Oyervides Garcia

Michael A Hall

Romain Fouquet

Jonathan C Lamb

Matthew Sean Marengo

Alana N Brown

Jeanette M Peevers

Kevin Lee Deppermann

Scott C Johnson

David V Butruille

Charles T Foresman

John A Miklos

Fenggao Dong

Bryce Lemke

Paul Cannon

Charles R Dietrich

Angela Ranae Koestel

Andrew M Singleton

Linda Rymarquis

Richard J Lawrence

Brad D White

Allen T Christian

William M Fischer

Huachun Wang Larue

Ruth A Wagner

Elias J Yannakakis

Charles L Armstrong

Michael Joseph Dayawon

Yang Ju Im

Fengxing Du

Jamaine R Hubbard

Thomas S Ream

G David Grothaus

Tiffany L Stephans

Donald W Mittanck

Yelena A Dudin

Ramesh Govinthasamy

Christopher T Bauer

Daniel Challis

Brian W Gardunia

Piyaraj Nan Newton

Pierre Sehabiague

Shengqiang Zhong

Ujwal A Deole

John F Lasinski

Amy D Trinchard

Josh G Rameaka

Chris Tierney

William A Grote

The present disclosure is in the field of plant breeding. The disclosure provides methods for breeding corn plants having a brachytic trait using marker-assisted selection. The disclosure further provides brachytic germplasm, markers associated with a brachytic trait for introgressing the trait into elite germplasm in a breeding program. This disclosure also provides brachytic or dwarf elite corn varieties having yield equal to or higher than conventional non-brachytic corn varieties.

Methods and compositions for producing brachytic corn plants

Methods for delayed harvesting of corn fields are provided herein. These methods provide an extended, flexible period of time to harvest corn. The methods allow growers to harvest their corn at the optimal time for drying down or accessing seed, without increasing the risk of losing yield to lodging.

Delayed harvest of short stature corn plants

Disclosed herein are plants exhibiting a semi-dwarf phenotype with reduced plant height compared to control wildtype plants. Some of the disclosed semi-dwarf plants comprise at least one non-natural brachytic mutation in which the activity of a BR2 gene is reduced. Also disclosed are methods for producing a semi-dwarf corn plant using a CRISPR based genome editing system.

Genome editing-based crop engineering and production of brachytic plants

Methods for improving the efficiency and productivity of hybrid corn seed production are provided herein. Various methods to improve the transfer of pollen from male corn plants to female corn plants, and thus increase yield, are provided herein. Without being limiting, these methods include varying the height of male and female corn plants in a field, as well as varying the number, arrangement, and ratio of male-to-female rows in a field.

Methods for hybrid corn seed production

Methods for providing compositions to corn fields prior to harvesting are provided herein. These methods provide an extended time period for the use of lower height or standard height farm equipment in-season in corn fields, while reducing the risk of damage to the corn plants. These methods also allow for late season access with lower height or standard height farm equipment, while reducing the risk of damage to the corn plants.

Management of corn through semi-dwarf systems

The invention provides novel methods to genotype haploid embryos using molecular assays. For example, quantitative methods for genotyping are provided. The methods provided also include providing a plurality of haploid kernels, determining the genotype of the haploid embryo of said kernels by distinguishing its genotype from the endosperm genotype, selecting a kernel having a desired genotype and producing doubled haploid plant from the selected kernel.

Methods for genotyping haploid embryos

Methods and compositions for improved plant breeding using gene editing and haploid induction are provided.

Methods and compositions for genome editing via haploid induction

An automated small particle distribution system is provided for transferring small particles from source tubes to destination tubes. The system includes a loading deck that is structured and operable to store and provide a plurality of source tube trays and a plurality of destination tube trays. Each source tube tray includes a plurality of source tubes stored therein, and each destination tube tray includes a plurality of destination tubes stored therein. The system additionally includes a work deck is structured and operable to receive selected source tube trays and selected destination tube trays from the loading deck, aspirate various specified amounts of small objects stored in selected source tubes, and deposit the aspirated small objects into selected destination tubes without cross-contamination of small objects.

Small object distribution automation

Portable systems and methods for amplifying nucleotides and for detecting nucleotide sequences in a sample are provided. The portable instruments and methods use RPA techniques for DNA amplification and detect sample fluorescence in response to amplification and/or to the presence of specific DNA sequences.

Portable systems and methods for amplifying nucleotides and detecting nucleotide sequences

Growing community of inventors

Chesterfield, MO, United States of America

Edward James Cargill