Applied Research Systems Ars Holding N.v.

Genzyme Corporation

Integrated Genetics, Inc.

Edward G Bernstine

Scott C Chappel

Nancy Hsiung

Anton K Beck

Vermuri B Reddy

Vermuni B Reddy

Biologically active heteropolymeric protein composed of a plurality of subunits, both subunits being synthesized in a single cell having an expression vector comprising heterologous DNA encoding the subunits. Preferably the protein is similar to the human or ungulate fertility hormones, LH and FSH.

Cells, vectors and methods for producing biologically active TSH

Described are new glycoprotein hormones capable of competing with natural hormones for the normal receptor binding sites but substantially incapable of effecting post receptor activities. The glycoprotein hormones of the present invention have had specific (rather than all) oligosaccharide chains removed so as to effectively diminish biologic activity while not significantly reducing plasma half-life, thus improving the molecules effectiveness as an antagonist compared with conventionally-produced molecules. The preferred glycoprotein hormones are ideally obtained by site-directed mutagenesis to selectively deglycosylate the protein. Also described are therapeutic treatments comprising the administration of the recombinant glycoprotein hormones of the present invention as hormone antagonists.

Site-directed mutagenesis modified glycoprotein hormones and methods of

Biologically active heterodimeric human fertility hormones composed of two different subunits, each subunit being synthesized in the same cell transformed by at least one cell expression vector having heterologous DNA encoding each subunit with each subunit being controlled by a separate promoter. Preferred human fertility hormones include hCG, hLH and hFSH.

Recombinant heterodimeric human fertility hormones, and methods, cells,

The correct sequence is disclosed for the beta subunit of human follicle stimulating hormone which, when present in a recombinant heterodimeric human fertility hormone allows the production of biologically active hormone. DNA coding for such beta subunit of human FSH may thus be prepared as well as expression vectors containing such DNA and cells transfected therewith.

DNA encoding the beta subunit of human follide stimulating hormone and

Site-directed mutagenesis modified DNA encoding glycoprotein hormones

Site-directed mutagenesis modified glycoprotein hormones

Biologically active heteropolymeric protein composed of a plurality of subunits, each subunit being synthesized by a cell having an expression vector heterologous DNA encoding the subunit.

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Boston, MA, United States of America

Edward G Bernstine