Beckman Coulter, Inc.

Terumo Bct, Inc.

Edward Allan Stanton

Paul Barclay Purcell

George C Malachowski

Kenneth Michael Evans

Jeffrey W Degeal

Charles S Bay

Thomas G Dilorenzo

In flow cytometry, particles () can be distinguished between populations () by combining n-dimensional parameter data, which may be derived from signal data from a particle, to mathematically achieve numerical results representative of an alteration (). An alteration may include a rotational alteration, a scaled alteration, or perhaps even a translational alteration. Alterations may enhance separation of data points which may provide real-time classification () of signal data corresponding to individual particles into one of at least two populations.

Enhancing flow cytometry discrimination with geometric transformation

A cell expansion system includes an air removal chamber to provide a bubble trap so that air and/or gas bubbles do not enter the bioreactor of the cell expansion system. The air removal chamber includes a pair of ports situated at the bottom of the air removal chamber. An entrance port allows fluid to enter the air removal chamber, and an exit port allows fluid to exit the air removal chamber. In one embodiment the air removal chamber forms an element of a premounted fluid conveyance assembly for use with a cell expansion machine.

Air removal chamber for a cell expansion system and method of use associated therewith

Disclosed are computer-implemented methods of sorting particles from a particle stream in a flow cytometer. The methods include: calculating sort decision making parameters using the raw event data values from a flow cytometer and a sort logic; performing sort logic computations using the sort logic definition and the sort decision making parameters to generate sort decisions; converting the sort decisions into sort commands; and sending the one or more sort commands to the flow cytometer. Sort logic computations may include algorithmically using conditional branching logic, and may include sort logic equations having mathematical functions characterizing one or more regions of interest in multidimensional data space. Such mathematical functions may be determined based on one or more parameters provided by a user. Also disclosed are corresponding systems having a flow cytometer and a computer.

Flow cytometer sorter

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Longmont, CO, United States of America

Edward Allan Stanton