Other

Rijksuniversiteit Groningen

Academisch Ziekenhuis Groningen

Eduardus Maria Dominicus Schuuring

Christian Bastard

Anthonie Willem Langerak

Michael Hummel

Ramon Garcia Sanz

Antonio Parreira

John Lewis Smith

Frances Louise Lavender

Gareth John Morgan

Paul Anthony Stuart Evans

Michael Kneba

Elizabeth Anne Macintyre

Jesus Fernando San Miquel

Gijsbertha Barendina Alida Wisman

Monika Brüggemann

Frederic Bernard Louis Davi

Jesus Fernando San Miguel

Ate Gerard Jan Van Der Zee

The invention relates to methods, reagents and kits for detecting the susceptibility to cervical cancer. In particular, it relates to novel methylation markers to improve screening for cervical intraepithelial neoplasia grade 2/3 (CIN2/3) and the use thereof for identifying a cervical cell as neoplastic or predisposed to neoplasia in an isolated sample.

Biomarkers for cervical cancer

The invention relates to PCR-based clonality studies for among others early diagnosis of lymphoproliferative disorders. Provided is a set of nucleic acid amplification primers comprising a forward primer, or a variant thereof, and a reverse primer, or a variant thereof, capable of amplifying a rearrangement selected from the group consisting of a VH-JH IGH rearrangement, a DH-JH IGH rearrangement, a VK-J&#x3ba; IGK rearrangement, a VK/intron-Kde IGK rearrangement, a V&#x3bb;-J&#x3bb; IGL rearrangement, a V&#x3b2;-J&#x3b2; TCRB rearrangement, a D&#x3b2;-J&#x3b2; TCRB rearrangement, a V&#x3b3;-J&#x3b3; TCRG rearrangement, a V&#x3b4;-J&#x3b4; TCRD rearrangement, a D&#x3b4;-D&#x3b4; TCRD rearrangement, a D&#x3b4;-J&#x3b4; TCRD rearrangement, a V&#x3b4;-D&#x3b4; TCRD rearrangement, or a translocation selected from t(11;14)(BCL1-IGH) and t(14;18)(BCL2-IGH). The primers can be used in PCR-based clonality studies for early diagnosis of lymphoproliferative disorders and detection of minimal residual disease (MRD). Also provided is a kit comprising at least one set of primers of the invention.

Nucleic acid amplification primers for PCR-based clonality studies

The invention relates to PCR-based clonality studies for among others early diagnosis of lymphoproliferative disorders. Provided is a set of nucleic acid amplification primers comprising a forward primer, or a variant thereof, and a reverse primer, or a variant thereof, capable of amplifying a rearrangement selected from the group consisting of a VH-JH IGH rearrangement, a DH-JH IGH rearrangement, a VK-JK IGK rearrangement, a VK/intron-Kde IGK rearrangement, a V&#x3bb;-J&#x3bb; IGL rearrangement, a V&#x3b2;-J&#x3b2; TCRB rearrangement, a D&#x3b2;-J&#x3b2; TCRB rearrangement, a V&#x3b3;-J&#x3b3; TCRG rearrangement, a V&#x3b4;-J&#x3b4; TCRD rearrangement, a D&#x3b4;-D&#x3b4; TCRD rearrangement, a D&#x3b4;-J&#x3b4; TCRD rearrangement, a V&#x3b4;-D&#x3b4; TCRD rearrangement, or a translocation selected from t(11;14)(BCL1-IGH) and t(14;18)(BCL2-IGH). The primers can be used in PCR-based clonality studies for early diagnosis of lymphoproliferative disorders and detection of minimal residual disease (MRD). Also provided is a kit comprising at least one set of primers of the invention.

Growing community of inventors

Groningen, Netherlands

Eduardus Maria Dominicus Schuuring