University of Rochester

University of Pennsylvania

Edith M Lord

Cameron J Koch

Ann R Kennedy

Xingsheng Wan

Mary A Taupier

Novel nitroaromatic compounds and immunogenic conjugates comprising a novel nitroaromatic compound and a carrier protein are disclosed. The invention further presents monoclonal antibodies highly specific for the claimed nitroaromatic compounds, the compounds' protein conjugates, the compounds' reductive byproducts, and adducts formed between the compounds and mammalian hypoxic cell tissue proteins. The invention is further directed to methods for detecting tissue hypoxia using immunohistological techniques, non-invasive nuclear medicinal methods, or nuclear magnetic resonance. Diagnostic kits useful in practicing the methods of claimed invention are also provided.

Detection of hypoxia

Monoclonal antibodies and a method capable of detecting Bowman Birk inhibitor metabolites in a sample of body fluid or tissue are provided.

Method of monitoring exposure to Bowman Birk inhibitor using monoclonal

Monoclonal antibodies and a method capable of detecting BBI metabolites in a sample of body fluid or tissue are provided.

Monoclonal antibodies differentially reactive with native and

Detection of hypoxia with reagents containing 2-nitroimidazole compounds

A serum free cell culture medium containing less than 10 ug/ml protein is disclosed. The medium comprises a basal media supplemented with levels of 2-aminoethanol substantially higher than previously disclosed and supplemented with 2-mercaptoethanol, transferrin, insulin and free essential amino acids. Unlike other serum free media, the media disclosed herein will support the growth and long term culture of a large variety of cell types including hybridomas and tumor cell lines while maintaining both growth potential and the differentiated characteristics of the particular cell line.

Growing community of inventors

Rochester, NY, United States of America

Edith M Lord