Plexium, Inc.

University of California

Celldom, Inc.

Pacific Biosciences of California, Inc.

Edgar Gutierrez

Arnold Oliphant

Jesse Lu

Eugene Tkachenko

Yi Zhang

Mark H Ginsberg

Alex Nemiroski

Alexander Groisman

Kapil Mahakalkar

Drew Ival Frerichs

Kenneth Chung

Natalia Tkachenko

Julian Sean Alberni

Kenneth Chng

Luke Daenitz

Provided are methods and compositions for cell-based drug screening with high-density arrays of conical microwells and slow-drug-release beads.

Device and method for cell-based drug screening

A method for fluid transport includes receiving fluid at an inlet port of an inlet. The fluid is outputted through an opening of the inlet into a channel. A first ratio of a first distance to a second distance is substantially equal to a cubic root of a second ratio between a first length dimension and a second length dimension of the inlet, the first distance being measured from an entrance of the inlet port to a first position within the inlet, the second distance being measured from the entrance of the inlet port to a second position within the inlet, the first length dimension and the second length dimension each being measured along a direction orthogonal to a measurement direction along the first distance and the second distance, the first length dimension and the second length dimension being measured at the first position and the second position, respectively.

Uniform fluid flow with constant resistance and velocity

The present invention provides methods, systems and kits for performing microwell analysis in sealed small volumes. The microwells of the invention are sealed through the pairing of microwell and bead geometry wherein the diameter of the beads is greater than the diameter of the bottom of the microwells. Loading the beads into the microwells seals samples within an analytical space between the bottom of a nested bead and the bottom of the subject microwell.

Sealed microwell assay

This disclosure provides for devices and methods for conduction assays for combinatorial libraries. The devices comprise a multiplicity of wells and a removable cap.

Caps for assay devices

A microfluidic system for fluid transport is provided. The microfluidic system includes a microfluidic device. The microfluidic device includes an inlet body including an inlet. The microfluidic device includes a base supporting the inlet body. The base includes a channel in fluid communication with the inlet. The base includes one or more sensors formed on a surface of the channel, or one or more sensors formed in one or more wells formed in the surface of the channel. The channel is configured to facilitate flow of the fluid. The fluid includes a plurality of beads. The fluid includes a plurality of suspended cells. The inlet is configured to receive the fluid at an inlet port. The inlet is configured to output the fluid through an opening in fluid communication with the channel. The inlet is configured to provide substantially uniform flow of the fluid across a substantial portion of a horizontal dimension of the channel. The device is configured to compensate for edge effects otherwise present therein. Related methods, apparatuses, systems, techniques and articles are also described.

Fluidic device

This disclosure provides for devices and methods for conducting assays for combinatorial libraries. The devices comprise a multiplicity of wells and a removable cap.

In a microfluidic system, a magnetic clamp for sealing a flexible microchannel chip includes a base formed from a magnetically-attractable material. The base supports a window for viewing the face of the microfluidic chip. A ring with magnets uniformly distributed around it is disposed over the base. A transparent disk attached to the top of the ring has an inlet and an outlet for introducing and removing a fluid medium into a cavity defined the disk, the window, the center opening of the ring and the base. An elastomer cushion is attached to the inner surface of the disk. The magnetic force between the base and the magnets on the ring compresses the cushion against the microfluidic chip so that the microchannels are sealed against the window with a uniform and reproducible pressure.

Growing community of inventors

San Diego, CA, United States of America

Edgar Gutierrez