Sanofi-a Ventis Deutschland Gmbh

Butalco Gmbh

Evonik Degussa Gmbh

Lesaffre Et Compagnie

Johann Wolfgang Goethe-universitat Frankfurt Am Main

Scandinavian Technology Group Ab

Lallemand Hungary Liquidity Management LLC

Sanofi-aventis Pharma Deutschland Gmbh

Gothia Yeast Solutions Ab

Cereduce Ab

Eckhard Boles

Silke Dlugai

Guenter Mueller

Mike Farwick

Steffen Schaffer

Christoph Schorsch

Heiko Andrea

Doerthe Voss

Dawid Brat

Marco Keller

Beate Wiedemann

Tim Köhler

Virginia Schadeweg

Mislav Oreb

Klaus-Peter Koller

Alexander Farwick

Beate Rother

Tim Koehler

Barbel Hahn-Hagerdal

Heiko Dietz

Kaisa Karhumaa

Lena Gustafsson

Daniel Wolff

Christer Larsson

Karin Elbing

Ferdinand Kirchner

Ansgar Poetsch

Roman Wieczorke

Jessica Becker

Martin Grininger

Roslyn Bill

Stefan Hohmann

Frank Ter Veld

Roman Niedbal

Gunter Muller

Renata Pavlovic

Jan Gajewski

Gunter Festel

Christian Weber

Marie-Francoise Gorwa-Grauslund

Thorsten Subtil

Baerbel Hahn-Haegerdal

The present invention relates to proteins involved in fatty acid synthesis, such as fatty acid synthases (FAS) variants, comprising one or more polypeptide chains, wherein said polypeptide chain(s) comprise one or more subunits comprising a malonyl/palmitoyl transferase domain (MPT domain), acetyl transferase domain (AT domain), and ketoacyl synthase domain (KS domain), and at least one amino acid substitution in the MPT domain at a position corresponding to R130, in the AT domain at a position corresponding to I306, and/or in the KS domain, preferably in the acyl binding channel and/or at KS domain binding site to ACP, to modulate affinities of acyl intermediates, and optionally further amino acid substitution(s). The present invention relates to the respective polypeptide domains. The present invention further relates to nucleic acid molecules encoding the proteins (or the polypeptide domains) and to host cells containing said nucleic acid molecules. The present invention further relates to a method for the production of short fatty acids, CoA esters of short fatty acids, ethyl esters of short fatty acids, esters of short fatty acids with other metabolites, and/or enzyme bound short fatty acids (Cto C), comprising the expression of said nucleic acid molecules, preferably in said host cells. The present invention further relates to a method for the production of biofuels, flavoring compounds and/or fine chemicals, comprising the expression of said nucleic acid molecules, preferably in said host cells. The present invention also relates to the use of the proteins, nucleic acids molecule or host cells for the bulk production of short fatty acids (Cto C), the specific production of Cfatty acids and/or C8 fatty acids, the bulk production of CoA esters of short fatty acids (Cto C), the specific production of C-CoA esters and/or C-CoA esters, the bulk production of ethyl esters of short fatty acids (Cto C), the specific production of Cfatty acid ethyl esters and/or Cfatty acid ethyl esters, the bulk production of esters of short fatty acids (Cto C) with other metabolites, the specific production of Cfatty acid esters with other metabolites and/or Cfatty acid esters with other metabolites, the bulk production of enzyme bound short fatty acids (Cto C), the specific production of enzyme bound Cfatty acids and/or enzyme bound Cfatty acids, the production of biofuels, fine chemicals and/or flavoring substances.

Microbiological production of short fatty acids and uses thereof

The present invention relates to polypeptides which are Gal2 variants comprising at least one amino acid substitution at a position corresponding to M435, and optionally further amino acid substitution(s). The present invention further relates to nucleic acid molecules encoding the polypeptides and to host cells containing said nucleic acid molecules. The present invention further relates to a method for the production of bioethanol and/or other bio-based compounds, comprising the expression of said nucleic acid molecules, preferably in said host cells. The present invention also relates to the use of the polypeptides, nucleic acids molecule or host cells for the production of bioethanol and/or other bio-based compounds, and/or for the recombinant fermentation of biomaterial containing pentose(s), preferably D-xylose and/or L-arabinose.

Variants of GAL2 transporter and their uses

The present invention relates to polypeptides which are Gal2 variants comprising at least one amino acid substitution at a position corresponding to T354, and optionally further amino acid substitution(s). The present invention further relates to nucleic acid molecules encoding the polypeptides and to host cells containing said nucleic acid molecules. The present invention further relates to a method for the production of bioethanol and/or other bio-based compounds, comprising the expression of said nucleic acid molecules, preferably in said host cells. The present invention also relates to the use of the polypeptides, nucleic acids molecule or host cells for the production of bioethanol and/or other bio-based compounds, and/or for the recombinant fermentation of biomaterial containing pentose(s), preferably D-xylose and/or L-arabinose.

Variants of Gal2 transporter and their uses

The present invention relates to the use of nucleic acid molecules coding for a bacterial xylose isomerase (XI), preferably coming from, for reaction/metabolization, particularly fermentation, of recombinant microorganisms of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol, by means of xylose fermenting yeasts. The present invention further relates to cells, particularly eukaryotic cells, which are transformed utilizing a nucleic acid expression construct which codes for a xylose isomerase, wherein the expression of the nucleic acid expression construct imparts to the cells the capability to directly isomerize xylose into xylulose. Said cells are preferably utilized for reaction/metabolization, particularly fermentation, of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol. The present invention also relates to methods for the production of bioethanol, and to methods for the production of further metabolization products, comprising the metabolization of media containing xylose.

Prokaryotic xylose isomerase for the construction of xylose-fermenting yeasts

The invention relates to genetically modifiedcells, to the use thereof and to a method of producing sphingoid bases and sphingolipids.

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The invention relates to novel enzymes that provide acetylated sphingoid bases.

Acetyltransferase from wickerhamomyces ciferrii

The present invention relates to novel expression cassettes and expression vectors, comprising three nucleic acid sequences for araA, araB and araD, each coding for a polypeptide of an L-arabinose metabolic pathway, in particular, a bacterial L-arabinose metabolic pathway. The invention particularly relates to expression cassettes and expression vectors, comprising codon-optimized nucleic acid sequences for araA, araB and araD. The invention further relates to host cells, in particular modified yeast strains containing the expression cassettes or expression vectors and expressing the polypeptides for the L-arabinose metabolic pathway, in particular, for the bacterial L-arabinose metabolic pathway. When using these modified host cells, arabinose is more effectively fermented by these cells, in particular into ethanol. The present invention is therefore relevant, inter alia, in connection with the production of biochemicals from biomass, such as bioethanol for example.

Vector with codon-optimised genes for an arabinose metabolic pathway for arabinose conversion in yeast for ethanol production

Prokaryotic xylose isomerase for the construction of xylose fermenting yeasts

The present invention relates to novel expression cassettes and expression vectors, comprising three nucleic acid sequences for araA, araB and araD, each coding for a polypeptide of an L-arabinose metabolic pathway, in particular, a bacterial L-arabinose metabolic pathway. The invention particularly relates to expression cassettes and expression vectors, comprising codon-optimised nucleic acid sequences for araA, araB and araD. The invention further relates to host cells, in particular modified yeast strains containing the expression cassettes or expression vectors and expressing the polypeptides for the L-arabinose metabolic pathway, in particular, for the bacterial L-arabinose metabolic pathway. When using these modified host cells, arabinose is more effectively fermented by these cells, in particular into ethanol. The present invention is therefore relevant, inter alia, in connection with the production of biochemicals from biomass, such as bioethanol for example.

The present invention relates to a method for producing a L-arabinose utilizing yeast strain for the production of ethanol, whereby a yeast strain is modified by introducing and expressing araA gene (L-arabinose isomerase), araB gene (L-ribulokinase D-N) and araD gene (L-ribulose-5-P 4-epimerase) and carrying additional mutations in its genome or overexpressing a TAL1 (transaldolase) gene, enabling it to consume L-arabinose, to use it as the only carbon source, and to produce ethanol, as well as a method for producing ethanol using such a modified strain.

Modified yeast consuming L-arabinose

The invention relates to a strain of the yeastwhich, owing to deletion of the genomic sequences, no longer synthesizes hexose transporters and, as a consequence, can no longer grow on substrates with hexoses as the only carbon source, and whose ability of growing on a substrate with a hexose as the only carbon source is restored when it expresses a GLUT4 gene.

The invention relates to a yeast cell producing isobutanol, characterized in that the cell has an increased metabolic flow of material from pyruvate and acetolactate, 2,3-dihydroxy isovalerate, 2-ketoisovalerate, isobutyraldehyde to isobutanol, in that at least one of the genes coding the enzymes, which are involved in this conversion, is over-expressed, and without any of said genes being heterologous to said yeast cell, and to a method for the production of isobutanol using yeast cells, comprising the provision of the yeast cells according to the invention, and bringing the yeast cell into contact with a fermentable carbon source.

Fermentative production of isobutanol with yeast

The invention relates to yeast strains in which a human GLUT4 transport or a human GLUT1 transporter can be functionally expressed and to particular GLUT4 transport proteins which can be functionally expressed particularly readily in yeast strains.

Astrain expressing both arabinose and xylose utilization pathways, and in particular to astrain fermenting both arabinose and xylose to ethanol, and more particularly astrain with overexpression or upregulation of xylose- or aldose reductase (XR, AR) with xylitol dehydrogenase (XDH) together with overexpression or upregulation of genes forming an arabinose utilization pathway. The invention encompasses both laboratory and industrial strain having these properties.

Growing community of inventors

Darmstadt, Germany

Eckhard Boles