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Eastern Virginia Medical School

Old Dominion University

E Stephen Buescher

Karl H Schoenbach

Stephen J Beebe

Peter F Blackmore

Methods for inducing calcium mobilization in cells through the application of nanosecond pulsed electric fields ('nsPEFs') are provided. The invention also provides a method of increasing intracellular calcium in cells through the application of nsPEFs. In one embodiment of the invention, the cells are human platelets, whereby activation and aggregation of the platelets is induced. Methods for treating an injury, trauma, or loss of blood in a subject, through the application of nsPEFs are also provided.

Activation of calcium-mediated cell functions in cells and tissues, including aggregation of human platelets. by nanosecond pulsed electric fields

A method for intracellular electro-manipulation is provided. The method includes applying at least one ultrashort electric field pulse to target cells. The ultrashort electric field pulse has sufficient amplitude and duration to modify subcellular structures in the target cells and does not exceed the breakdown field of the medium containing the target cells. The amplitude and duration of the ultrashort electric field pulse are typically insufficient to substantially alter permeability of the surface membranes of the target cells, e.g., by irreversibly disrupting the cell surface membranes. An apparatus for intracellular electro-manipulation is also provided. The apparatus includes a pulse generator capable of producing an ultrashort electric pulse output and a delivery system capable of directing the electric pulse output to target cells.

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Virginia Beach, VA, United States of America

E Stephen Buescher