Viacyte, Inc.

University of Georgia Research Foundation Inc

E Edward Baetge

Kevin Allen D'Amour

Alan D Agulnick

Chad Green

Xiaojie Yu

Thomas C Schulz

Allan J Robins

Olivia Kelly

Anne Bang

Eugene Brandon

Stephen Dalton

Allan Sheppard

Karen Jones

Kevin A D'amour

The present invention relates to methods for production of undifferentiated or differentiated embryonic stem cell aggregate suspension cultures from undifferentiated or differentiated embryonic stem cell single cell suspensions and methods of differentiation thereof.

Stem cell aggregate suspension compositions and methods of differentiation thereof

The present invention provides compositions and methods for the production of differentiated mammalian cells. More particularly, the present invention provides cellular differentiation methods employing culturing the cells on a feeder layer or under feeder-free conditions in cell culture and further contacting the cells with an inhibitor of the PI3-kinase pathway and a member of the TGFb family for the generation of differentiated mammalian cells from pluripotent mammalian stem cells. Preferably, the differentiated cell is selected from the group consisting of a mesendodermal cell, a mesodermal cell, and an endodermal cell.

Methods for increasing definitive endoderm differentiation of pluripotent human embryonic stem cells with PI-3 kinase inhibitors

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Encinitas, CA, United States of America

E Edward Baetge