Lexicon Pharmaceuticals, Inc.

The United States of America, as Represented by the Secretary, Department of Health and Human Services

E-Chiang Lee

Gregory M Landes

Seokjoo Hong

David Reed Powell

Urvi Desai

Ling Chen

Xiao Feng

Kyu Chung

Donald L Court

Daiguan Yu

Hilary M Ellis

Nancy A Jenkins

Neal G Copeland

Shyam K Sharan

Monoclonal antibodies that specifically bind to ANGPTL3 are provided. Monoclonal antibodies that neutralize at least one activity of ANGPTL3 are provided. Methods of treating a disorder of lipid metabolism using neutralizing monoclonal antibodies are provided.

Monoclonal antibodies against ANGPTL3

Monoclonal antibodies that specifically bind to ANGPTL4 are provided. Monoclonal antibodies that neutralize at least one activity of ANGPTL4 are provided. Methods of treating a disorder of lipid metabolism using neutralizing monoclonal antibodies are provided.

Monoclonal antibodies against ANGPTL4

Disclosed herein are methods for generating recombinant DNA molecules in cells using homologous recombination mediated by recombinases and similar proteins. The methods promote high efficiency homologous recombination in bacterial cells, and in eukaryotic cells such as mammalian cells. The methods are useful for cloning, the generation of transgenic and knockout animals, and gene replacement. The methods are also useful for subcloning large DNA fragments without the need for restriction enzymes. The methods are also useful for repairing single or multiple base mutations to wild type or creating specific mutations in the genome. Also disclosed are bacterial strains and vectors which are useful for high-efficiency homologous recombination.

Enhanced homologous recombination mediated by lambda recombination proteins

Growing community of inventors

The Woodlands, TX, United States of America

E-Chiang Lee