University of Washington

Dylan Gary Karis

Alshakim Nelson

Ashleigh Brooks Theberge

Rose Ann Cattolico

Siyami Millik

Patrick Smith

Robert Jun Ono

Ashley Dostie

Chloe Ramos Deodato

Abhijit Saha

Trevor Gerald Johnston

Methods of fabricating a hydrogel tube, and related systems, employ extrusion of a cross-linkable hydrogel solution from an annular outer nozzle of a nozzle assembly to form a cross-linkable hydrogel tube. The cross-linkable hydrogel tube is cured to form a cross-linked hydrogel tube. A second hydrogel solution can be coextruded via the axial inner nozzle to form an inner hydrogel filament coaxially positioned within the cross-linkable hydrogel tube. The cross-linked hydrogel tube can be functionalized with collagen to enable cell adhesion, and cells can be cultured on the luminal surfaces of these tubes to yield tubular endothelial layers. A 3D printed coaxial nozzle can be used to fabricate biofunctional tubular conduits that can be utilized for engineering in vitro models of tubular biological structures.

Extruded hydrogel tubes and coaxial fibers and applications thereof

Hydrogel compositions including a polymer uniformly embedded with a loading agent are provided. Also provided are methods for extrusion printing hydrogel compositions to provide extruded hydrogel compositions, which can be crosslinked to provide crosslinked hydrogel structures. Also provided are methods for using crosslinked hydrogel structures in chemical processes.

3D printable hydrogel materials

Growing community of inventors

Seattle, WA, United States of America

Dylan Gary Karis