Celemics, Inc.

Yonsei University

Duhee Bang

Hyojun Han

Hwangbeom Kim

Sangun Park

Jeewon Lee

Joongoo Lee

Nam Jin Cho

Namjin Cho

Han Na Seo

Hyeonseob Lim

Eui Jin Kwon

A method for retrieving nucleic acid fragments, including: providing reduced DNA libraries during sequencing; amplifying nucleic acid fragments from the reduced DNA libraries; and retrieving the amplified nucleic acid fragments. The method further includes acquiring desired nucleic acid fragments among the retrieved nucleic acid fragments. The desired nucleic acid fragments are acquired using sequences having a homology to the barcode sequences, when the amplified nucleic acid fragments are tagged with specific barcode sequences.

Methods for retrieving sequence-verified nucleic acid fragments and apparatuses for amplifying sequence verified nucleic acid fragments

Disclosed is a method of preparing nucleic acid molecules, including: providing a pool of oligonucleotides, each containing restriction enzyme digestion sequences and generic flanking sequences; cleaving the restriction enzyme digestion sequence portions to provide a pool of mixtures comprising the oligonucleotides, each containing the generic flanking sequences at one end, and the oligonucleotides, each containing none of the generic flanking sequences at one end, and; assembling the oligonucleotides using the generic flanking sequences to randomly synthesize nucleic acid fragments.

Method of preparing nucleic acid molecules

Provided is a method for stably synthesizing an error-free gene using a high-depth oligonucleotide tiling, which includes designing an oligonucleotide fragment by an over-overlapping method, synthesizing the oligonucleotide fragment using DNA microarray, retrieving error-free oligonucleotide fragments retrieved by next generation sequencing, and assembling the error-free oligonucleotide fragments.

Method for synthesizing gene using high-depth oligonucleotide tiling

Proposed is a method of easily finding an error during analysis of various library sequences of nucleic acid base sequences after synthesizing a gene library using a combination of three nucleic acid base sequences (codon) translated into the same protein. This shows that it is possible to create a gene library having the same protein sequence but different nucleic acid base sequences. The present disclosure provides a novel experimental method capable of measuring a correlation between gene expression according to change in a codon by changing the usage of a codon optimized to express a specific gene in vivo.

Method of synthesis of gene library using codon randomization and mutagenesis

Provided is a method of preparing nucleic acid molecules comprising: (a) a step of providing nucleic acid fragments constituting at least a portion of the complete sequence of a target nucleic acid; (b) tagging the nucleic acid fragments with barcode sequences; (c) identifying the sequence of the nucleic acid fragments tagged by the barcode sequences; and (d) recovering desired nucleic acid fragments among the sequence-identified nucleic acid fragments using the barcode sequences.

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Seoul, South Korea

Duhee Bang