Bio-rad Laboratories, Inc.

Other

Affymetrix, Inc.

Pacific Biosciences of California, Inc.

Biomerieux, Inc.

Do, Duc

Litterst, Claudia

Maar, Dianna

Duc Do

Claudia Litterst

Dianna Maar

Charles Garrett Miyada

Kevin L Gunderson

Gene W Shen

John Lyle

Lubomir Sebo

Thomas R Gingeras

Andrei Fedorov

Kai Wu

Ali Laayoun

Qing Yang

Xiangxu Kong

Austin So

Robert Joseph Weber

Stephen P Dudek

Carsten Rosenow

Fred C Christians

Grace Lee

Austin P So

Charles Garret Miyada

Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification.

Digital assays with a generic reporter

Digital assay system, includes methods, apparatus, and compositions, for distinguishing and measuring different types of templates according to the different lengths of corresponding amplicons, which may be amplified by the same pair of primers. The system may include a length-sensitive reporter generating luminescence that varies according to amplicon length. The system may, for example, be utilized to identify, distinguish, and/or quantify wild-type and mutant/variant templates, processed and unprocessed template, a target template and a primer dimer, or the like.

Digital assays with a reporter for amplicon length

Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photo-induced damage mitigating agents to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.

Photo-induced damage mitigating agents and preparation and methods of use thereof

The presently claimed invention provides methods, compositions, and apparatus for studying nucleic acids. Specifically, the present invention provides a novel enrichment and labeling strategy for ribonucleic acids. In one embodiment, the invention provides enriching for a population of interest in a complex population by diminishing the presence of a target sequence. In a further embodiment, the invention can be used to reproducibly label and detect extremely small amounts of nucleic acids.

Preparation of nucleic acid samples

A process is provided for labeling with signal amplification a ribonucleic acid (RNA), comprising fragmenting the RNA to form RNA fragments, fixing a first ligand to a terminal phosphate located at least one of the 3&prime; end and the 5&prime; end of each of a plurality of the RNA fragments, the terminal phosphate having been released during the fragmentation, and binding a plurality of labeling agents to the first ligand on each of a plurality of the fragments.

Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby

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San Jose, CA, United States of America

Duc Do