Intercell Ag

Valneva Austria Gmbh

Duc Bui Minh

Andreas Meinke

Claire M Fraser

Eszter Nagy

Wolfgang Zauner

Christoph Klade

Oresta Vytvytska

Hildegard Etz

Agnieszka Dryla

Martin Hafner

Uwe Von Ahsen

Steven Gill

Tamas Henics

Thomas Weichhart

Brigitte Tempelmaier

Brigitre Tempelmaier

Described is a method for identification, isolation and production of hyperimmune serum-reactive antigens from a specific pathogen, a tumor, an allergen or a tissue or host prone to auto-immunity, said antigens being suited for use in a vaccine for a given type of animal or for humans, which is characterized by the following steps: providing an antibody preparation, providing at least one expression library, identifying antigens which bind, screening the identified antigens, identifying the hyperimmune serum-reactive antigen portion of said identified antigens and which hyperimmune serum-reactive antigens bind to a relevant portion of said individual antibody optionally isolating said hyperimmune serum-reactive antigens and producing said hyperimmune serum-reactive antigens by chemical or recombinant methods.

Method for identification, isolation and production of antigens to a specific pathogen

Described is a method for identification, isolation and production of hyperimmune serum-reactive antigens from a specific pathogen, a tumor, an allergen or a tissue or host prone to autoimmunity, said antigens being suited for use in a vaccine for a given type of animal or for humans, which is characterized by the following steps:&#x2014;providing an antibody preparation from a plasma pool of said given type of animal or from a human plasma pool or individual sera with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity,&#x2014;providing at least one expression library of said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity,&#x2014;screening said at least one expression library with said antibody preparation,&#x2014;identifying antigens which bind in said screening to antibodies in said antibody preparation,&#x2014;screening the identified antigens with individual antibody preparations from individual sera from individuals with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity,&#x2014;identifying the hyperimmune serum-reactive antigen portion of said identified antigens and which hyperimmune serum-reactive antigens bind to a relevant portion of said individual antibody preparations from said individual sera and&#x2014;optionally isolating said hyperimmune serum-reactive antigens and producing said hyperimmune serum-reactive antigens by chemical or recombinant methods.

Described is a method for identification, isolation and production of hyperimmune serum-reactive antigens from a specific pathogen, a tumor, an allergen or a tissue or host prone to autoimmunity, said antigens being suited for use in a vaccine for a given type of animal or for humans, which is characterized by the following steps: &#x2014;providing an antibody preparation from a plasma pool of said given type of animal or from a human plasma pool or individual sera with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity, &#x2014;providing at least one expression library of said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity, &#x2014;screening said at least one expression library with said antibody preparation, &#x2014;identifying antigens which bind in said screening to antibodies in said antibody preparation, &#x2014;screening the identified antigens with individual antibody preparations from individual sera from individuals with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity, &#x2014;identifying the hyperimmune serum-reactive antigen portion of said identified antigens and which hyperimmune serum-reactive antigens bind to a relevant portion of said individual antibody preparations from said individual sera and &#x2014;optionally isolating said hyperimmune serum-reactive antigens and producing said hyperimmune serum-reactive antigens by chemical or recombinant methods.

Growing community of inventors

Vienna, Austria

Duc Bui Minh