Chinese Academy of Sciences

Universitat Fur Bodenkultur Wien

Guangzhou Institute of Biomedicine and Health

Guangzhou Healthquest Pharma Co., Ltd.

Hefei Castem Cell and Regenerative Medicine Co., Ltd.

Guangzhou Institute of Biomedicine and Health, Chines Academy of Sciences

Duanqing Pei

Lihui Wang

Zhang Zhang

Tao Wang

Jing Liu

Ke Ding

Johannes Grillari

Regina Grillari

Yanmei Zhang

Jiekai Chen

Pengfei Liu

Deping Wang

Mengjie Shen

Kun Luo

Yubing Feng

Linli Wang

Yanting Xue

Guangjin Pan

Miguel Esteban

Shubin Chen

Xingguo Liu

Ting Zhou

Jinglei Cai

Meixiu Peng

Jinglei Liu

Deyang Yao

Xuebin Liu

Provided are a culture medium for preparing neural stem cell and use thereof, the culture medium for preparing neural stem cell comprising: a basic culture medium suitable for the growth of stem cell, and a cell signal pathway inhibitor selected from at least one of GSK inhibitor, MEK inhibitor, TGF-&#x3b2; inhibitor, ROCK inhibitor and BMP inhibitor.

Culture medium for preparing neural stem cells and use thereof

Methods for generating iPSCs and differentiated cells of interest by reprogramming donor cells that have been obtained in a non-invasive manner. In particular, the donor cells are exfoliated epithelial urine cells. The differentiated cells can be obtained by differentiation of the reprogrammed iPSCs or by direct reprogramming the urine cells.

Method of generating induced pluripotent stem cells and differentiated cells

The usage of a stem cell in preparation of a tooth-like structure is provided. And a culture medium, a method for preparing an epithelial-like cell, a kit for preparing an ameloblast, a method for preparing an ameloblast are also provided. Specifically, the culture medium comprises a basal medium, which is DMEM/F12 medium; N2 supplement; retinoic acid; and BMP-4.

Preparing tooth-like structure using stem cell

The present invention relates to a method for increasing the efficiency of inducing pluripotent stem cells by utilizing genes Jhdm1b and Jhdm1a that modify histone. By utilizing Jhdm1b, Jhdm1a, and a stem cell inducing factor, the present invention increases the efficiency of inducing pluripotent stem cells and increases the quality of induced pluripotent stem cells. The stem cell inducing factor is a combination of Oct4 and Klf4, or a combination of Sox2, Oct4, and Klf4, or a combination of Oct4 and Sox2, and Oct4 alone. The method further comprises exposing the cells to vitamin C, which further increases the efficiency of inducing pluripotent stem cells as compared with the case where no vitamin C is used. By using less stem cell reducing factors, the method of the present invention reduces the potential carcinogenicity, obtains a high inducing efficiency, and provides high-quality induced pluripotent stem cells capable of germ-line transmission.

Method for increasing the efficiency of inducing pluripotent stem cells

The present invention relates to a method for increasing the efficiency of inducing pluripotent stem cells by utilizing genes Jhdm1a that modify histone. By utilizing Jhdm1a, and a stem cell inducing factor, the present invention increases the efficiency of inducing pluripotent stem cells and increases the quality of induced pluripotent stem cells. The stem cell inducing factor is a combination of Oct4 and Klf4, or a combination of Sox2, Oct4, and Klf4, or a combination of Oct4 and Sox2, and Oct4 alone. The method further comprises exposing the cells to vitamin C, which further increases the efficiency of inducing pluripotent stem cells as compared with the case where no vitamin C is used. By using less stem cell reducing factors, the method of the present invention reduces the potential carcinogenicity, obtains a high inducing efficiency, and provides high-quality induced pluripotent stem cells capable of germ-line transmission.

The heterocyclic alkynyl benzene compounds of formula (I), their pharmaceutically acceptable salts and stereoisomers thereof, as well as application in preparing drugs for preventing or treating tumors. The compounds can overcome the clinically induced resistance against Gleevec. 

Heterocyclic alkynyl benzene compounds and medical compositions and uses thereof

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Guangzhou, China

Duanqing Pei