University of California

Duane E Keith, Jr

Robert H Edwards

Christopher J Evans

Daniel L Kaufman

Recombinant forms of isolated and purified delta opioid receptors and methods to screen for compounds interactive with delta opioid receptors are described.

DNA molecules encoding opioid receptors and methods of use thereof

Genes encoding opioid receptors (including opioid-like receptor (ORL) proteins) can be retrieved from vertebrate libraries using the murine probe disclosed herein under low-stringency conditions. The DNA sequence shown inor its complement can be used to obtain the human delta, kappa and mu genes as well as the murine mu gene and human ORL-1. The probe provided encodes the murine delta opioid receptor.

Opioid receptors and methods of use

Genes encoding opioid receptors (including opioid-like receptor (ORL) proteins) can be retrieved from vertebrate libraries using the murine probe disclosed herein under low-stringency conditions. The DNA sequence shown in FIG. 5 or its complement can be used to obtain the human delta, kappa and mu genes as well as the murine mu gene and human ORL-1. The probe provided encodes the murine delta opioid receptor.

Methods of screening modulators of opioid receptor activity

Genes encoding opioid receptors can be retrieved from vertebrate libraries using the murine probe disclosed herein under low-stringency conditions. The DNA sequence shown in FIG.,or its complement can be used to obtain the human delta, kappa and mu genes as well as the murine mu gene. The probe provided encodes the murine delta opioid receptor.

Opioid receptor genes

Nucleic acid molecules comprising a nucleotide sequence encoding a mammalian delta opioid receptor are disclosed. The invention provides recombinant materials for the production of mammalian delta opioid receptors.

Growing community of inventors

Woodland Hills, CA, United States of America

Duane E Keith, Jr