The United States of America, as Represented by the Secretary, Department of Health and Human Services

Drew C Deniger

Steven A Rosenberg

Paul F Robbins

Yong-Chen Lu

Maria R Parkhurst

Anna Pasetto

Steven A Feldman

Rami Yoseph

Parisa Malekzadeh

Winifred M Lo

Disclosed are methods of selectively expanding a number of T cells. The methods may comprise: modifying human T cells to express a TCR, wherein the TCR comprises a murine constant region; producing a population of cells comprising a number of human T cells expressing the TCR and a number of human T cells not expressing the TCR; and culturing the population of cells in the presence of (i) irradiated feeder cells, (ii) one or more cytokines, and (iii) an antibody, or an antigen-binding portion thereof, wherein the antibody has antigenic specificity for the murine constant region of the TCR, so as to selectively expand the number of T cells expressing the TCR over the number of T cells not expressing the TCR. Also disclosed are related populations of cells, pharmaceutical compositions, and methods of treating or preventing cancer.

Methods for selectively expanding cells expressing a TCR with a murine constant region

Disclosed is an isolated or purified T cell receptor (TCR) having antigenic specificity for mutated human p53. Related polypeptides and proteins, as well as related nucleic acids, recombinant expression vectors, host cells, populations of cells, and pharmaceutical compositions are also provided. Also disclosed are methods of detecting the presence of cancer in a mammal and methods of treating or preventing cancer in a mammal.

T cell receptors recognizing mutated P53

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Houston, TX, United States of America

Drew C Deniger