Contipro A.s.

Contipro Pharma A.s.

Drahomira Chladkova

Vladimir Velebny

Radovan Buffa

Ivana Basarabova

Gloria Huerta-Angeles

Jaromir Kulhanek

Katerina Knotkova

Josef Chmelar

Hana Vagnerova

Sofiane Kettou

Lucie Pospisilova

Helena Krejci

Lubos Sobotka

Martina Hermannova

Tomás Pitucha

Martin Sojka

Tomas Pitucha

A chlorinated derivative of hyaluronic acid or of a modified hyaluronic acid (chloramide) is provided. The chloramide has an amidic group (&#x2014;NH&#x2014;CO&#x2014;). The hydrogen of the amidic group is substituted by a chlorine atom, according to the structural formula &#x2014;NCl&#x2014;CO&#x2014;. The substitution degree of the hyaluronic acid or of the modified hyaluronic acid by chlorine is in an amount of from 50 to 100 %.

Chlorinated derivative of hyaluronic acid, method of preparation thereof, a composition containing the derivative, and use thereof

The invention relates to a new method of preparation of a hyaluronan derivative with an aldehydic group in the position (6) of the polysaccharide glucosamine part. The hyaluronic acid oxidation can be performed by means of TEMPO/NaCIO or TEMPO/TCC systems in a protic environment with or without the presence of anorganic salts. Thus prepared aldehyde can be used for binding amines, diamines, amino acids, peptides and other compounds containing an amino group, e.g. by means of the reductive amination with NaBHCN in water or in a water-organic solvent system. When a diamine or compounds containing three or more amino groups are used, cross-linked hyaluronan derivatives can be prepared. Cross-linked derivatives can be also prepared by a reaction of the aldehyde with a hyaluronan substituted by an amino-alkyl group HA-alkyl-NH.

Method of preparation of an oxidized derivative of hyaluronic acid and a method of modification thereof

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Letohrad, Czechia

Drahomira Chladkova