Transgenomic, Inc.

Phynexus, Inc.

Other

Sarasep, Inc.

Cetac Technologies, Inc.

Vosbio, Inc.

Gjerde, Douglas T.

Douglas T Gjerde

Paul D Taylor

Robert M Haefele

Christopher P Hanna

Lee Hoang

Chris Suh

David W Togami

Guenther Bonn

Ronald Jones

Allen Burge

Mark J Abel

Christopher T Hanna

David P Hornby

Carrie Loan Kim Huynh

Christian Wolfgang Huck

Karl H Hecker

Kimberly A Lamb

Thomas Jakschitz

Jonathan Michael Grambow

John E Sutton

Raquel R Roque

Jennifer E Grant

Alexander I Kuklin

Robert M Hae Fele

Benjamin L Legendre, Jr

Mathias Rainer

Daniel R Wiederin

Günther Bonn

Leon Yengoyan

Rania Bakry

James V Benson

Matthias Rainer

Isabel Feuerstein

David Peter Joseph Hornby

Jeffrey L Sklar

Fabio Polato

Laura Bunnel

Satyajit Kar

Maryam Matin

Arezou Azarani

Gunther Stecher

Alexander Bonn

Bernhard Maerk

Alezander I Kuklin

Harald Sonderegger

Guenther Stecher

Leon S Yengoyan

A water solvated chromatography bed is packed into the distal end of a syringe barrel. The syringe pumping piston is modified to be flat to match the top frit structure (that is also flat). The piston pump is pushed into the syringe barrel until the piston is directly above the column top frit. The pumping piston is moved forth quickly to dislodge entrained air in the bed. The piston pump moves directly above the resin bed with no air gap above the bed.

Piston pump syringe column method and device

The present invention relates to a method for qualitative analysis of a sample protein, which method comprises the steps of providing a water swollen gel column bed comprising bound protease and a sample protein in liquid buffer, digesting the sample protein into polypeptides by contact with the gel bed and subjecting the polypeptides to mass spectrometry (MS). The method is advantageously performed using back and forth flow of the sample protein including two or more repeats, and may be performed at a temperature of less than about 37° C., such as a temperature of less than about 30° C. The invention also includes an automated method as well as a device and a kit for performing mass-based analysis of proteins with higher speed than the prior art while maintaining conditions that are tolerable to the protease.

Qualitative analysis of proteins

This invention employs solid phase extraction media and column methods to apply external and internal standards and compounds. Analytical standard or compounds including PFAS are adsorbed to a solid phase extraction media and are stored indefinitely. The standards or compounds remain stable on the solid phase extraction media without decomposing. The standards or compounds may be removed from the solid phase extraction media with an elution solvent or reagent.

Columns and methods of use for analytical standards and compounds

Methods, devices and kits for sampling, releasing and stabilizing nucleic acid, including RNA and DNA, from virus, bacteria yeast and other cells is described. The released and stabilized nucleic acid may be analyzed and quantified without further sample preparation at the point of care or may be transported to a testing laboratory by shipment and analyzed directly. The nucleic acid, which can be RNA, remains safe and stable so that shipping by normal means including government postal service may be used. In addition, the RNA sample remains stable so that analysis can be performed immediately after receipt of sample or after storage for days, weeks or months. Storage may be at room or ambient temperature or cooler temperatures. The sampling apparatus used to acquire samples can interface with nucleic detection and measurement instrumentation including high throughput, parallel processing instruments.

Methods, devices and kits for preparing nucleic acid samples for storage and analysis

This invention employs columns and methods to apply external and internal standards and compounds. Analytical standard or compounds are adsorbed to a solid phase extraction media and are stored indefinitely. The standards or compounds remain stable on the solid phase extraction media without decomposing. The standards or compounds may be removed from the solid phase extraction media with a solvent.

The invention provides columns (including pipette tip columns) and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.

Devices and methods for plasmid purification

This invention relates to devices and methods for purifying, detecting and using biological cells. A variety of cell types including viable tumor, stem, immune and sperm cells can be purified from a complex biological sample using a column, including a pipette tip column. Methods of the invention can aid research, diagnosis and treatment of cancer. Purified viable cells can be detected on the column or eluted from the column and detected. Cells on a column can be used as a stationary phase for liquid chromatography. Cells may be removed, recovered and analyzed.

Isolation, detection and use of biological cells

Columns for isolation, detection and use of biological cells

This invention relates to devices and methods for purifying biological cells. For example, viable tumor, stem, immune and sperm cells can be purified from a complex biological sample using a pipette tip column. Methods of the invention can aid research, diagnosis and treatment of cancer.

Methods for purifying biological cells

The invention provides pipette tip columns and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned in the pipette tip column, above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.

Methods and devices for nucleic acid purification

Growing community of inventors

Saratoga, CA, United States of America

Douglas T Gjerde