University of California

Molecular Express, Inc.

Merck Sharp + Dohme Corp.

Douglas S Watson

Francis C Szoka, Jr

Gary Fujii

Kathryn M Story

Mark C Sleevi

Allison D Ortigosa

Michael A Rauscher

Francis C Szoka

Allison C Ortigosa

The present invention relates to liposomal vaccine compositions, methods for the manufacture thereof, and methods for the use thereof to stimulate an immune response in an animal. These compositions comprise dimyristoylphosphatidylcholine ('DMPC'); either dimyristoylphosphatidylglycerol ('DMPG') or dimyristoyltrimethylammonium propane ('DMTAP') or both DMPC and DMTAP; and at least one sterol derivative providing a covalent anchor for one or more immunogenic polypeptide(s) or carbohydrate(s).

Methods and compositions for liposomal formulation of antigens and uses thereof

A process is described for purifying insulin and insulin analogs that comprises use of two or more orthogonal chromatographic purification steps in tandem following enzymatic digestion of the propeptide-insulin precursor to remove specific product impurities, improve process consistency, and increase process redundancy in the purification of the insulin or insulin analog, e.g., insulin lispro.

Chromatography process for purification of insulin and insulin analogs

A method for crystallizing insulin or insulin analogs under alkaline conditions in the presence of zinc, purifying the crystals by filtering through a filter, and drying the crystals captured on the filter to produce crystalline insulin or insulin analog compositions is described. Method has been exemplified with insulin lispro.

Method for preparing crystalline insulin or insulin analog compositions

The present invention relates to liposomal vaccine compositions, methods for the manufacture thereof, and methods for the use thereof to stimulate an immune response in an animal. These compositions comprise dimyristoylphosphatidylcholine ('DMPC'); either dimyristoylphosphatidylglycerol ('DMPG') or dimyristoyltrimethylammonium propane (&#x201c;DMTAP&#x201d;) or both DMPC and DMTAP; and at least one sterol derivative providing a covalent anchor for one or more immunogenic polypeptide(s) or carbohydrate(s).

Methods and compositions for liposomal formulation  of antigens and uses thereof

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Harrisonburg, VA, United States of America

Douglas S Watson