University of California

Other

Rensselaer Polytechnic Institute

USA As Represented by Secretary of the Navy

University System of Maryland

Bp Corporation North America Inc.

Albany Molecular Research, Inc.

University of Iowa Research Foundation

Darpa

Enzymed, Inc.

Douglas S Clark

Jonathan S Dordick

David Curtis Turner

Banahalli R Ratna

Paul E Schoen

Michael A Markowitz

Bruce P Gaber

Harvey W Blanch

Paul Roger Kust

Yuri L Khmelnitsky

John F Hartwig

F Dean Toste

Peter C Michels

Joseph B Binder

Sanil Sreekumar

Frank Thomson Robb

Jay D Keasling

Jungbae Kim

David H Sherman

Jing Huang

Amit A Gokhale

Aindrila Mukhopadhyay

Aravind Srinivasan

Gorkem Gunbas

Pongpan Laksanalamai

Melinda E Clark

Pawel Dydio

Cheryl L Budde

John M Arnold

Alexander Ya Usyatinsky

Sharon S Chen

Joseph O Rich

Yuri Khmelnitsky

Victor Overby

Zhennan Liu

Hanna M Key

Anchalee Jiemjit

Timothy Whitehead

Joel Edward Graham

Tae-Wan Kim

Harshal Akshay Chokhawala

Konstantinos A Goulas

Zachary C Baer

Zach Baer

Paul A Willems

Pazhamalai Anbarasan

Frank Thomas Robb

The present invention provides for a genetically modified host cell capable of producing an unnatural compound, such as terpenoid, comprising a P450 enzyme comprising an artificial cofactor, and the host cell is capable of uptaking the artificial cofactor by comprising a heterologous heme transport transmembrane protein, or homologue thereof.

Host cells and methods useful for producing unnatural terpenoids using a novel artificial metalloenzyme

The present invention is drawn to artificial metalloenzymes for use in cyclopropanation reactions, amination and C&#x2014;H insertion.

Artificial metalloenzymes containing noble metal-porphyrins

The present disclosure provides methods to produce ketones suitable for use as fuels and lubricants by catalytic conversion of an acetone-butanol-ethanol (ABE) fermentation product mixture that can be derived from biomass.

Method to convert fermentation mixture into fuels

The present disclosure generally relates to the catalytic conversion of alcohols into hydrocarbon ketones suitable for use as fuels. More specifically, the present disclosure relates to the catalytic conversion of a mixture of isopropanol-butanol-ethanol (IBE) or acetone-butanol-ethanol (ABE), into ketones suitable for use as fuels. The ABE or IBE mixtures may be obtained from the fermentation of biomass or sugars.

Methods to produce fuels

The present disclosure relates to mutant thermostable glycosyl hydrolases family 7 enzymes, including mutantendoglucanase I. In particular, the present disclosure relates to mutant thermostable enzymes, compositions containing the enzymes, and methods of use thereof.

[object Object]

Hydrolysis and degradation of cellulose-containing biomass by use of a polypeptide having cellulase activity is provided. Also provided are polypeptides having cellulase activity, such as archaeal cellulases, polynucleotides encoding the polypeptides, and compositions containing the polypeptides, and methods of use thereof.

Polypeptides for use in the deconstruction of cellulose

The invention provides methods and compositions for increasing tolerance of microorganisms to toxic agents, such as solvents; and for increasing production of solvents from solvent-generating microorganisms. The methods comprise engineering a microorganism of interest to express a heterologous heat-shock protein/chaperone, e.g., Group II chaperonin or a prefoldin such as &#x3b3;-prefoldin, where the heterologous protein is from an extremophile, such as an archaean.

Heterologous expression of extremophile heat shock proteins and chaperones in microorganisms to increase tolerance to toxic compounds

A system and method for conducting high-throughput interactions between test compositions and analytes, comprising one or more test compositions, and a plurality of independent micromatrices, wherein each said micromatrix encapsulates at least one said test composition; and said micromatrices are made of a material that is permeable to an analyte.

Biocatalytic solgel microarrays

Disclosed herein is a microfluidics device that can be used to prepare natural products and their analogs. The device comprises the enzymes of a biosynthetic pathway immobilized thereon and a means for sequentially directing a starting material and each ensuing reaction product to the enzymes of the biosynthetic pathway in the order corresponding to the steps of the biosynthetic pathway. The device can thus be used to prepare the natural product using the natural starting material of the biosynthetic pathway or analogs of the natural product using an unnatural starting material. Alternatively, artificial pathways can be created by immobilizing an appropriate selection of enzymes on the device in an order whereby each subsequent enzyme can catalyze a reaction with the product of the prior enzyme. Novel chemical entities can be prepared from these artificial pathways.

In vitro metabolic engineering on microscale devices

A method of making a molecularly imprinted porous structure makes use of a surfactant analog of the molecule to be imprinted that has the imprint molecule portion serving as the surfactant headgroup. The surfactant analog is allowed to self-assemble in a mixture to create at least one supramolecular structure having exposed imprint groups. The imprinted porous structure is formed by adding reactive monomers to the mixture and allowing the monomers to polymerize, with the supramolecular structure serving as a template. The resulting solid structure has a shape that is complementary to the shape of the supramolecular structure and has cavities that are the mirror image of the imprint group. Similarly, molecularly imprinted particles may be made by using the surfactant to create a water-in-oil microemulsion wherein the imprint groups are exposed to the water phase. When reactive monomers are allowed to polymerize in the water phase to form particles, the surface of the particles have cavities that are the mirror image of the imprint group.

Molecularly-imprinted material made by template-directed synthesis

Highly specific biocatalytic reactions have been used to create a population of taxol derivatives.

Taxol derivatives

Growing community of inventors

Orinda, CA, United States of America

Douglas S Clark