Georgetown University

Douglas M Gersten

Robert S Ledley

Edward J Zapolski

A method of metabolically mapping one or more central nervous system components of a mammalian body by computerized axial tomography scanning which entails injecting an effective amount of a 2-, 5- or 6-iodo-substituted hexose, as a contrast medium, into the mammalian body, and scanning the mammalian body by computerized axial tomography, thereby visualizing the concentration flux of said hexose in the central nervous system component or components.

Radioopaque sugar derivatives and a method of metabolic mapping using

Radioactive staining of gels is employed to identify proteins. A radioactive stain composition is formed by introducing radioactive iron isotope into ferrous bathophenanthroline sulfonate by adding crystalline ascorbic acid and acetic acid to a .sup.59 FeCl.sub.3 solution, adding aqueous BPS solution after the dissolution of the crystals, and adding methanol and acetic acid to yield the radioactive stain composition. Alternatively, the radioactive stain composition may be formed by bombarding a non-radioactive stain with thermal neutrons. The method of identifying proteins with the radioactive stain composition basically comprises the steps of placing the protein in the gel, forming a radioactic stain composition, and applying the radioactivated stain composition to the gel. To demonstrate the protein present, either a gamma scintillation counting technique or radioautography is preferably employed.

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Washington, DC, United States of America

Douglas M Gersten